Amin Kazemizadeh; Ahmad Zare Shahneh; Ali Reza Yousefi; Hasan Mehrabani Yeganeh; Zarbakht Ansari Pirsaraei
Volume 20, Issue 3 , November 2018, , Pages 487-498
Abstract
The present study was conducted to investigate the effect of dietary Curcumin supplementation on histological parameters of testis in aged Ross 308 broiler breeder roosters. A total of twelve 48-week old broiler breeder roosters in a completely randomized design were randomly assigned to four treatments ...
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The present study was conducted to investigate the effect of dietary Curcumin supplementation on histological parameters of testis in aged Ross 308 broiler breeder roosters. A total of twelve 48-week old broiler breeder roosters in a completely randomized design were randomly assigned to four treatments and three replicates during a 13 weeks of experimental period. Treatments included no dietary Curcumin supplementation (control group), and daily supplementation of 10, 20, and 30 mg Curcumin/birds as mixed in the basal diet. At the end of the experimental period, all of the roosters were slaughtered, and testis tissue samples were collected. Testicular weight was higher in the roosters that daily received 30 mg Curcumin compared with the control group (P<0.05). Dietary supplementation of 20 and 30 mg Curcumin/day increased the diameter of seminiferous tubule compared to the control group (P<0.05). Seminiferous epithelium thickness was dose-dependently increased in Curcumin-supplemented birds compared to the control group (P<0.05). The number of spermatogonia cells was increased in all treatment groups compared to the control group (P<0.05). The number of Leydig cells was also increased in roosters received 20 and 20 mg Curcumin/day compared to the control birds (P<0.05). However, treatments did not affect the number of testis blood vessels (P>0.05). According to the results of the present study, dietary supplementation of 30 mg Curcumin/day/bird improves testis histological parameters in aged broiler breeder roosters.
Raana Jahanbin; Parisa Yazdanshenas; Mehdi Amin Afshar; Abdollah Mohammadi Sangcheshmeh; Hamid Varnaseri; Mohammad Chamani; Mohammad Hasan Nazaran; Mohammad Reza Bakhtiyarizadeh
Volume 17, Issue 2 , October 2015, , Pages 371-380
Abstract
The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, ...
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The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc Nano-complex and frozen. After thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. The proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. Zinc Nano-complex groups represented a higher plasma membrane functionality than that of control group. Moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc Nano-complex possessed higher mitochondrial activity as compared with the control group. Mitochondrial activity in 10-2 was higher than the 10-6 group. In conclusion, supplementation of zn Nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters.