Zabihollah nemati; magsoud besharati; amir karimi
Volume 21, Issue 1 , April 2019, , Pages 49-60
Abstract
The current research was designed to evaluate the effect of dietary addition of garlic powder, Cornerian cherry fruit powder (CH) and a mixture essential oil of castor and cashew nut shell (EO) on performance, egg quality and some blood parameters in commercial laying hens. One hundred and twenty-eight ...
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The current research was designed to evaluate the effect of dietary addition of garlic powder, Cornerian cherry fruit powder (CH) and a mixture essential oil of castor and cashew nut shell (EO) on performance, egg quality and some blood parameters in commercial laying hens. One hundred and twenty-eight 58-wk-old LSL laying hens were used in 4 treatments with 4 replicates and 8 birds per each in a completely randomized design. The experimental diets included: 1) Basal diet; 2) Basal diet +1% garlic powder; 3) Basal diet+1% CH and 4) Basal diet+0.15 percent EO. The experimental diets were fed from 59 to 63 weeks of age. The productive performance was recorded from 61 to 63 weeks of age and blood and egg yolk parameters measured at the end of the experimental period. There were no significant differences in laying hen performance among all treatments. Albumen height and Haugh unit was increased in CH compared to the control group (p<0.05). The blood cholesterol, HDL, triglyceride and aspartate aminotransferase and alanine aminotransferase enzymes did not show any significant difference among treatments. The garlic and CH powders significantly decreased egg yolk cholesterol concentration (p<0.05), however it was not significantly affected by the EO. Finally, it was concluded that addition of CH and garlic powder in the diet of laying hens could improve the egg quality and reduce yolk cholesterol concentrations.
mehdi Vafaye valleh; nahid Karimi zandi; Farzaneh Bazmandegan Shomeyli
Volume 20, Issue 4 , February 2019, , Pages 513-525
Abstract
In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross ...
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In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross 308 eggs were randomly assigned into two equal experimental groups including test (In ovo injection of Khazak yolk) and control (In ovo injection of Ross yolk) group. After hatching, chickens were reared for six weeks under the same standard environmental conditions with exposure to some certain inflammatory stimuli between 21-28 days of age. Chicken’s blood and/or tissues samples were collected on days 10 and 42, and the samples were analyzed for the target traits. Results showed that Khazak yolk component caused a reduction in the levels of pro-inflammatory TNF-α cytokine in both offspring's liver and intestinal tissue (P<0.05). Compared to the control group, khazak yolk injection was found to enhance the titer of IgA natural antibodyas well as primary and secondary antibody titer response against sheep red blood cells (SRBC) and reduces both serum levels of offspring's CRP protein and liver ALT enzyme (P<0.05). According to the results of the present study, injection of Khazak native hen egg-yolk into the yolk of eggs from Ross 308 commercial broiler breeder can effectively suppress the expression of TNF-α inflammatory cytokine in the offspring's liver and intestinal tissue.
Farzaneh Bazmandegan
Volume 19, Issue 2 , August 2017, , Pages 507-519
Abstract
To examine the possible role of maternal effect on progeny immune system development, the injection effects of the khazak yolk into the yolk of the commercial Ross egg on the performance, immunity and expression of TLR4 was determined in the commercial Ross chicken. For this purpose, 150 ROSS fertile ...
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To examine the possible role of maternal effect on progeny immune system development, the injection effects of the khazak yolk into the yolk of the commercial Ross egg on the performance, immunity and expression of TLR4 was determined in the commercial Ross chicken. For this purpose, 150 ROSS fertile eggs were randomly assigned to two experimental groups including group I (control-in ovo Ross yolk injection) and group II (in ovo khazak yolk injection) and were kept in incubator for 3 weeks. After incubation period, newly hatched chickens were fed with balanced ration for six weeks. Three Chickens from each experimental group were respectively killed at 27- and 42-d posthatch for analysis of immune organs weight (liver, burs and thymus), The HI anti- body titer levels and liver TLR4 mRNA expression levels. The results of Statistical analysis demonstrated that the in-ovo injection of khazak yolk into the Ross eggs not only significantly enhances growth rate and immune function but also decreases expression of TLR4 mRNA in the liver of treated group compared with the control group (P˂0.05). On the basis of these results it's possible that injection of khazak yolk into the Ross eggs at the first day of embryonic development enhances the chicken immune response at older ages.