Maryam Mohammadnezhad; Ghodrat Rahimi Mianji; Ayoub Farhadi
Volume 23, Issue 1 , March 2021, , Pages 1-11
Abstract
The aim of this study was to identify the allelic variants of C4-A and LF genes and to investigate their associations with the nunber of milk somatic cells in Holstein cows. Mastitis is one of the most common diseases in dairy cows, that reduces milk production and imposes high costs on breeders. C4-A ...
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The aim of this study was to identify the allelic variants of C4-A and LF genes and to investigate their associations with the nunber of milk somatic cells in Holstein cows. Mastitis is one of the most common diseases in dairy cows, that reduces milk production and imposes high costs on breeders. C4-A and lactoferrin genes are amonge the genes that affect the immune system to fight pathogens, microbial agents and mastitis. 384 blood samples were prepared from Holstein cows and DNA extraction was performed using optimized salting out method. IPLEX technique was used to determine the genotype of the samples. To identify single nucleotide polymorphisms, the marker sites of 1-rs137485678; C<G and 2-rs132741478; C<T for C4- A complement gene and 1-rs384176726; A<G and 2-rs445918028; A<T were selected for the LF gene. Genotyping at 1-rs137485678; C<G locus of C4-A gene showed two C and G alleles with frequencies of 58 and 42 %, and also three genotypes CC, CG and GG with frequencies of 31, 53 and 16%, respectively. No polymorphisms were observed at the other sites. Marker-trait analysis showed a statistically significant association (P<0.05), so that cows with CG genotype had the lowest number of somatic cells. Phase software was used to identify haplotypes. Based on the results of this research, the marker rs137485678; C <G-1 of the C4-A gene can be used as a genetic marker to improve mastitis in dairy cows.
Jafar Pish Jang Aghajeri; Ghodrat Rahimi Mianji; Hasan Hafezian; Mohsen Gholizadeh
Volume 20, Issue 4 , February 2019, , Pages 527-537
Abstract
In the present study, the polymorphism of four loci including of TLR4, IL2, IgL and IFNγ, in 100 commercial broiler and layer chickens using PCR-RFLP technique and enzymes of Tsp509I, Sau96I and HphI were investigated. In TLR4 marker site, two alleles of C (two bands of 138 and 119 bp) and G (three ...
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In the present study, the polymorphism of four loci including of TLR4, IL2, IgL and IFNγ, in 100 commercial broiler and layer chickens using PCR-RFLP technique and enzymes of Tsp509I, Sau96I and HphI were investigated. In TLR4 marker site, two alleles of C (two bands of 138 and 119 bp) and G (three bands of 119, 99 and 39 bp), In IL2 locus, two alleles of A (four bands of 465, 64, 40 and 31bp) and B (five bands of 454, 64, 40, 31 and 11 bp), in IgL locus, two alleles M (two bands of 173, 161 and two bands of 10 bp) and N (three bands of 161, 103,70 and two bands of 10 bp) and in IFNγ locus, two alleles E (one band of 129 bp) and F (two bands of 90 and 39 bp) were identified. In TLR4, IL2, IgL and IFNγ markers sites, the population structure of the total population including the Shannon information index 0.43, 0.62, 0.68 and 0.69, the Fixation index -0.19, 0.37, -0.18 and 0.17 and the observed heterozygosity index 0.32, 0.27, 0.58 and 0.41 were estimated, respectively. Regarding the presence of polymorphism at these sites and in the case of studying the immune responses of the observed genotypes for these sites, can be used as marker for genetic breeding programs of chickens for increase of resistance to disease.