Mohammad Abdoli; Mohammad Bagher Zandi; Taher harkinezhad; Masoud Kahlili
Volume 23, Issue 2 , July 2021, , Pages 155-163
Abstract
The aim of this study was to investigate the genetic structure of Iranian native horse breeds to compare genetic diversity and understanding the relationships between the populations using 11 ISAG microsatellite markers. For this reason, 565 samples of the Iranian Equestrian database from different ages ...
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The aim of this study was to investigate the genetic structure of Iranian native horse breeds to compare genetic diversity and understanding the relationships between the populations using 11 ISAG microsatellite markers. For this reason, 565 samples of the Iranian Equestrian database from different ages including the Iranian Arab Asil, Caspian, Darehshouri, Kurdish and Turkmen and 113 samples were used for each breed. The number of observed alleles for each locus was 7 to 19 alleles with an average of 10.81 alleles, and it was 19 for ASB17 and 7 for HTG4 locus with the highest and lowest observed alleles ranking respectively. The average observed heterozygosity from the largest to the lowest rank was, Turkmen (0.68±0.11), Caspian (0.67±0.07), Kurdish (0.66±0.06) Darehshouri (0.65±0.07), and Arab Asil (0.62±0.08). Population structure analysis with UPGMA method showed that Caspian and Kurdish populations were grouped as a unit cluster while the other populations grouped as a separate cluster. These results confirmed this hypothesis that the Caspian and Kurdish populations are close to the Nisa horses. In general, the results of this study indicate that the Iranian native horses have got a high genetic diversity, despite of populations have genetic similarity and the other hand genetic clustering of the populations is consistent with their geographic distances. The result of this study shows that the ISAG microsatellite markers are polymorphic and have more efficiency for assignment genetic diversity and genetic structure analysis of Iranian native horse breeds.
Somayeh Zamani afshar; Taher harkinezhad; abas Bahari; mohamad hossein shahir
Volume 20, Issue 2 , August 2018, , Pages 213-224
Abstract
Selection of live animals with minimum carcass fat in animal breeding programs will lead to increase in meat production at national level. The ligands of Wnt genes are of the effective factors in adipocyte cell differentiation. This study was aimed to assess the association between polymorphisms in Wnt10a ...
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Selection of live animals with minimum carcass fat in animal breeding programs will lead to increase in meat production at national level. The ligands of Wnt genes are of the effective factors in adipocyte cell differentiation. This study was aimed to assess the association between polymorphisms in Wnt10a and Wnt10b genes and carcass traits in 96 Afshari – Booroola Merino male lambs. In this study, DNA was extracted from blood samples using phenol-chloroform extraction method and polymerase chain reaction was performed for amplification a 663 bp fragment of exon III and a part of the second intron of Wnt10a gene and a 512 bp fragment of exon III Wnt10b gene. The results showed a polymorphism and three monomorphisms (a different allele compared to reference sequence of the gene) in the Wnt10a gene but all the sequences of the Wnt10b fragment were the same in studied region of the gene. Results of the sequencing lead to identification of four single nucleotide changes in Wnt10a gene in the studied area when compared to the reference sequence. One of the monomorphisms was in the intron and among other three nucleotide changes identified in exon III one was a missense in codon 139. After digestion with restriction enzymes HpaII at this codon, it was observed that all sampled lambs had mutant homozygous genotype compared to the reference sequence. This site was also evaluated in a number of Afshari (fat- tailed) and Zell (with the least fat tail) but again the same results were observed. Therefore, it seems that in this position the G is the wild type allele in the studied population.
Mohammad Hossein Nemati; Mohammad Hossein Shahir; Mohammad Taher Harakinezhad; Houshang Lotfollahian
Volume 15, Issue 1 , July 2014, , Pages 45-53
Abstract
This experiment was conducted to determine the effect of vitamin C (VC) and coenzyme Q10 (CoQ10) onimmunity response of broilers in cold stress condition using 500 male chicks in a completely randomizeddesign with 5 treatments and five replicates. Treatments were: positive control (PC, normal conditionsgrowth ...
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This experiment was conducted to determine the effect of vitamin C (VC) and coenzyme Q10 (CoQ10) onimmunity response of broilers in cold stress condition using 500 male chicks in a completely randomizeddesign with 5 treatments and five replicates. Treatments were: positive control (PC, normal conditionsgrowth without antioxidants), negative control (NC, cold stress without antioxidants), cold stress + VC(300 mg/kg diet), cold stress + CoQ10 (40 mg/kg diet) and cold stress + VC + CoQ10 at above mentioneddoses. To induce cold stress, from day 15 until end, temperature fixed at 15oC. Vaccine titers, humeraland cellular immune response and relative immune organs weight were studied. Results showed that coldstress decreased relative spleen weight but there was no significant difference between treatments.Relative weight of bursa was increased in cold stress condition (P<0.01) and using antioxidant especiallyCoQ10 decreased it (P<0.05). Lymphocyte percent decreased in cold stress (P<0.05). Improved cellmediatedimmune response to subcutaneous injection of phytohemagglutinin and proliferation of Tlymphocyte in vitro were observed (P<0.05). In conclusion, use of antioxidants VC and CoQ10 in the coldstress condition improved immune system activity and decreased mortality