F. Pourbayramian; M. Ghaderzadeh; H. A. Deljoo Isaloo; P. Biabani; M. B. Shams Borhan; P. Barenj Foroush
Volume 14, Issue 2 , January 2012, , Pages 21-30
Abstract
The current study was designed to estimate the frequency of IGF-I gene 1 polymorphism and investigate if their polymorphisms have association with biometric traits in Moghani sheep by PCR-SSCP method. For this purpose, a number of 100 sheep were randomly chosen from Moghani sheep Jafarabad, Moghan breeding ...
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The current study was designed to estimate the frequency of IGF-I gene 1 polymorphism and investigate if their polymorphisms have association with biometric traits in Moghani sheep by PCR-SSCP method. For this purpose, a number of 100 sheep were randomly chosen from Moghani sheep Jafarabad, Moghan breeding station that located in Ardabil state and blood samples were collected from the left jugular vein using vacuum tubes. DNA were extracted from blood samples that using the salting-out procedure and amplified a fragment of 279 bp in size from IGF-I gene exon 1. The Single-Strand Conformation Polymorphism (SSCP) method was used for genotyping. Electrophoresis of PCR products were done on acrylamide gel that observed the polymorphic in this region. Based on SSCP patterns, three genotypes BC, BD and BB obtained with frequencies of 65, 26 and nine percent, respectively. The genotypes in this locus deviated from HWE. The traits data were analyzed using the SAS program. The BB genotype were associated with Leg girth but no association of the other genotypes with the withers height, back height, body length, breast girth other examined biometric traits were found (P< 0.05).