Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X

The effect of thyme and cinnamon essential oils on performance, rumen fermentation profile and rumen microbial population under in vitro and in vivo conditions

Document Type : Research Paper

Authors

Behzad Khorrami 1
Seyed Alireza Vakili 2
mohsen danesh mesgaran 3

1 Corresponding Author, Department of Animal and Poultry Science, Faculty of Agricultural Technology, College of Agriculture & Natural Resources, University of Tehran, Pakdasht, Iran. E-mail: bkhorrami@ut.ac.ir

2 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. E-mail: sarvakili@um.ac.ir

3 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. E-mail: danesh@um.ac.ir

https://doi.org/10.22059/jap.2024.375614.623792
Abstract
Introduction: Today, the use of antimicrobial agents such as antibiotics in livestock diets is facing reduced social acceptance because of the appearance of residues in milk and meat, and therefore, ruminant nutritionists are seeking for appropriate alternatives for these feed additives. The study of essential oils (EOs) as natural feed additives capable of improving rumen microbial fermentation has taken into consideration by nutritionists. Among the EOs, thyme (THY) and cinnamon (CIN) oil have attracted significant attention due to their antimicrobial activity against ruminal microorganisms. Therefore, more research is required to understand their impacts on rumen microbial fermentation in ruminants. Moreover, to the best of our knowledge, few studies has synchronously compared the influences of THY and CIN on rumen fermentation and microorganisms. This study was conducted to evaluate the effect of THY and CIN on performance, and rumen microbial fermentation under in vitro and in vivo conditions.
Materials and methods: In the in vitro experiment, different concentrations (0, 125, 250, 500, and 1000 mg/l) of THY and CIN were incubated for 24-h in batch culture. In the in vivo study 18 growing Holstein calves were used in a completely randomized design to examine effects of supplementing a basal diet (CON) with thyme oil (THY, 5 g/d/calf) or cinnamon oil (CIN, 5 g/d/calf) on performance and rumen microbial fermentation. Calves were fed ad libitum diets consisting of 15% forage and 85% concentrate for 80 d.
Results and discussion: The high concentration of THY and CIN (1000 mg/l) inhibited rumen microbial fermentation thereby decreasing total gas production and DM disappearance at 24 of incubation, and total VFA concentration. Cinnamon oil at 1000 mg/l increased final pH of batch culture (P<0.05). The lower concentration of THY and CIN (125 and 250 mg/l) decreased the acetate to propionate ratio (P<0.05), without reducing VFA concentration. Supplementation of THY or CIN did not affect DMI and ADG. There were also no effects of EOs on ruminal pH, rumen concentrations of ammonia nitrogen and total VFA; whereas molar proportion of acetate and ratio of acetate to propionate decreased (P<0.05), and the molar proportion of propionate increased (P<0.05). Rumen molar proportion of butyrate was significantly increased (P<0.05) by adding CIN. The population of protozoa and methanogens bacteria decreased in the rumen of calves receiving EOs (P<0.05). Ruminal population of Fibrobacter succinogenes was not affected by treatments, but populations of Ruminococcus albus and flavefaciens decreased by THY and CIN (P<0.05). 
Conclusion: Based on the results of the present study, although dietary addition of THY and CIN did not have a significant effect on the performance of feedlot calves, but it improved the rumen fermentation properties, which may be considered as potential alternatives for antibiotics in beef production systems.  

Keywords

Cinnamon oil
Feedlot calves
In vitro gas production
Rumen microbial fermentation
Thyme oil
رحمتی‌زاده، مرضیه؛ هژبری، فردین؛ و کفیل‌زاده، فرخ (1401). تأثیر افزودن مخلوطی از اسانس‌های نعناع فلفلی، آویشن و رزماری به جیره بر عملکرد رشد، فراسنجه‌های تخمیر شکمبه و متابولیت‌های خون بره‌های پرواری. علوم دامی ایران، 53 (4)، 285-273.
 
References
Anassori, E., Dalir-Naghaden, B., Pirmohammadi, R., Taghizedeh, A., Asri-Rezaei, S., Maham, M., Farahmand-Azar, S., & Farhoomand, P. (2011). Garlic: A potential alternative for monensin as a rumen modifier. Livestock Science, 142, 276-287.
Benchaar, C. (2021). Diet supplementation with thyme oil and its main component thymol failed to favorably alter rumen fermentation, improve nutrient utilization, or enhance milk production in dairy cows. Journal of Dairy Science, 104, 324-336.
Benchaar, C., Lettat, A., Hassanat, F., Yang, W. Z., Forster, R. J., Petit, H. V., & Chouinard, P. Y. (2012). Eugenol for dairy cows fed low or high concentrate diets: Effects on digestion, ruminal fermentation characteristics, rumen microbial populations and milk fatty acid profile. Animal Feed Science and Technology, 178, 139-150.
Busquet, M., Calsamiglia, S., Ferret, A., & Kamel, C. (2006). Plant extracts affect in vitro rumen microbial fermentation. Journal of Dairy Science, 89, 761-771.
Calsamiglia, S., Busquet, M., Cardozo, P., Castillejos, L., & Ferret, F. (2007). Essential oils as modifiers of rumen microbial fermentation: a review. Journal of Dairy Science, 90, 2580-2595.
Cardozo, P. W., Calsamiglia, S., Ferret, A., & Kamel, C. (2006). Effects of alfalfa extract, anise, capsicum, and a mixture of cinnamaldehyde and eugenol on ruminal fermentation and protein degradation in beef heifers fed a high-concentrate diet. Journal of Animal Science, 84, 2801-2808.
Castillejos, L., Calsamiglia, S., & Ferret, A. (2006). Effect of essential oils active compounds on rumen microbial fermentation and nutrient flow in in vitro systems. Journal of Dairy Science, 89, 2649-2658.
Castro Filho, E. S., Júnior, L. C. R., Ezequiel, J. M. B., Salles, M. S. V., Almeida, M. T. C., Perez, H. L., & van Cleef, E. H. C. B. (2021). Effect of thyme essential oil supplementation on feed intake, apparent digestibility, rumen fermentation, blood parameters and in vitro methane yield of Nellore cattle. Livestock Science, 244, 104349.
Chaves, A.V., He, M.L., Zang, W.Z., Hristov, A.N., McAllister, T.A., & Benchaar, C. (2008b). Effects of essential oils on proteolytic, deaminative and methanogenic activities of mixed ruminal bacteria. Canadian Journal of Animal Science, 88, 117-122.
Chaves, A.V., Stanford, K., Dugan, M. E. R., Gibson, L. L., McAllister, T. A., Van Herk, F., & Benchaar, C. (2008a). Effects of cinnamaldehyde, garlic and juniper berry essential oils on rumen fermentation, blood metabolites, growth performance, and carcass characteristics of growing lambs. Livestock Science,117, 215-224.
Cieslak, A., Szumacher-Strabel, M., Stochmal, A., & Oleszek, W. (2013). Plant components with specific activities against rumen Methanogens. Animal, 7, 253-265.
Fandino, I., Calsamiglia, S., Ferret, A., & Blanch, M. (2008). Anise and capsicum as alternatives to monensin to modify rumen fermentation in beef heifers fed a high concentrate diet. Animal Feed Science and Technology, 145, 409-417.
Fraser, G. R., Chaves, A. V., Wang, Y., McAllister, T. A., Beauchemin, K. A., & Benchaar, C. (2007). Assessment of the effects of cinnamon leaf oil on rumen microbial fermentation using two continuous culture systems. Journal of Dairy Science, 90, 2315-2328.
Helander, I. M., Alakomi, H. L., Latva-Kala, K., Mattila-Sandholm, T., Pol, L., Smid, E. J., Gorris, L. G. M., & von Wright, A. (1998). Characterization of the action of selected essential oil components on gram negative bacteria. Journal of Agricultural and Food Chemistry, 46, 3590-3595.
Jahani-Azizabadi, H., Danesh Mesgaran, M., Vakili, A. R., Rezayazdi, K., & Hashemi, M. (2011). Effect of various medicinal plant essential oils obtained from semi-arid climate on rumen fermentation characteristics of a high forage diet using in vitro batch culture. African Journal of Microbiology Research, 5, 4812-4819.
Klieve, A. V., & Hegarty, R. S. (1999). Opportunities for biological control of ruminal methanogenesis. Australian Journal of Agricultural Research, 50, 1315-1319.
Lin, B., Lu, Y., Salem, A. Z. M., Wang, J. H., & Liang, Q. J. X. (2013). Effects of essential oil combinations on sheep ruminal fermentation and digestibility of a diet with fumarate included. Animal Feed Science and Technology, 184, 24-32.
Macheboeuf, D., Morgavi, D. P., Papon, Y., Mousset, J. L., & Arturo-Schaan, M. (2008). Dose-response effects of essential oils on in vitro fermentation activity of the rumen microbial population. Animal Feed Science and Technology, 145, 335-350.
McIntosh, F. M., Williams, P., Losa, R., Wallace, R. J., Beever, D. A., & Newbold, C. J. (2003). Effects of essential oils on ruminal microorganisms and their protein metabolism. Applied and Environmental Microbiology, 69, 5011-5014.
Menke, K. H., & Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal research and development, 28, 7-55.
Moss, A. R., Jouany, J. P., & Newbold, J. (2000). Methane production by ruminants: its contribution to global warming. Review article. Annales de zootechnie, 49, 231-253.
Orzuna-Orzuna, G. F., Dorantes-Iturbide, G., Lara-Bueno, A. Miranda-Romero, L. A., Mendoza-Martínez, G. D., & Santiago-Figueroa, I. (2023). Meta-Analysis of Essential Oils Use for Beef Cattle Feed: Rumen Fermentation, Blood Metabolites, Meat Quality, Performance and, Environmental and Economic Impact. Fermentation, 8, 254.
Rahmatizadeh, M., Hozhabri, F., & Kafilzadeh, F. (2023). The effect of adding a mixture of peppermint, thyme and rosemary essential oils to diet on growth performance, rumen fermentation parameters and blood metabolites of fattening lambs. Iranian Journal of Animal Science, 53(4), 273-285. (In persian).
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and no starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583-3597.
Yang, W. Z., Ametaj, B. N., Benchaar, C., & Beauchemin, K. A. (2010a). Dose response to cinnamaldehyde supplementation in growing beef heifers: ruminal and intestinal digestion. Journal of Animal Science, 88, 680-688.
Yang, W. Z., Ametaj, B. N., Benchaar, C., & Beauchemin, K. A. (2010b). Cinnamaldehyde in feedlot cattle diets: intake, growth performance, carcass characteristics, and blood metabolites. Journal of Animal Science, 88, 1082-1092.
Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X
Volume 26, Issue 3
Autumn 2024
Pages 263-277

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