Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X

Comparison of the antioxidant effect of olive leaf extract with vitamins C and E on bovine sperm quality

Document Type : Research Paper

Author

Arash Kheradmand
https://doi.org/10.22059/jap.2016.59220
Abstract
In this study antioxidant effects of olive leaf extract (OLE) in comparison to vitamin C and E on chilled-stored (at 5 °C) bovine sperm was evaluated. The sperm samples collected from epididymis of ten Holstein bovine testis with 3-5 years old, and were diluted with egg yolk and sodium citrate containing 0.5, 1.0 and 2.0 mg/ml of OLE as well as 2 mg/ml vitamin E and C. Addition of OLE significantly reduced sperm motility (P<0.05). Vitamin E significantly improved sperm membrane integrity compared to the other groups (P<0.05). TBARS in 2 mg/ml of OLE treatment was significantly less than that in other treatments (P<0.05). The results showed that addition of OLE to bovine sperms stored at 5 ͦ C for 48 h negatively affected sperm motility despite of a decrease in lipid peroxidation, and therefore, it is not suitable extender to be used for bovine spermatozoa.

Keywords

Bovine
Lipid Peroxidation
Oleuropein
Sperm
Thiobarbituric acid reactive substances
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Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X
Volume 19, Issue 1
Spring 2017
Pages 233-244

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