Arezoo Tooghi; Gholam Dashab; Ali Maghsoudi; Mohammad Rokouei
Volume 24, Issue 2 , July 2022, , Pages 127-138
Abstract
The aim of this study was to investigate the genetic diversity of different camel breeds in Sistan and Baluchestan province using microsatellite markers. For this purpose, 70 camels were randomly selected from Jamazeh and Baluchi breeds. Jugular vein blood sampling was performed in the tubes containing ...
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The aim of this study was to investigate the genetic diversity of different camel breeds in Sistan and Baluchestan province using microsatellite markers. For this purpose, 70 camels were randomly selected from Jamazeh and Baluchi breeds. Jugular vein blood sampling was performed in the tubes containing 0.5% EDTA. The extraction of DNA was performed by salt-detergent method. Five microsatellite markers were selected from the camel genome project with specific primers and banding patterns on 5% agarose gel. Genetic and population structures were analyzed using POPGENE v1.31 software. The number of alleles in five marker positions in Jamazeh and Baluchi breeds ranged from one to six alleles. The number of alleles in five markers CVRLO6, VOLP32, LCV66, WYLLO and CMS17 in Jamazeh are equal to three, two, three, four and one alleles, respectively, and in Baluchi breed with two, four, two, five and one alleles, respectively. According to the results, the range of heterozygosity observed in the studied sites in Jamazeh and Baluchi breeds was in the range from 0.29 to 0.52. The results of FST analysis showed that the two populations were more than 96% similar and the difference between them was 3%. The results of the presentresearchindicated a low rate of polymorphism in the studied sites and in order to preserve breeds and be more adaptable to environmental conditions, it needs more attention and control of inbreeding.
Mohammad Abdoli; Mohammad Bagher Zandi; Taher harkinezhad; Masoud Kahlili
Volume 23, Issue 2 , July 2021, , Pages 155-163
Abstract
The aim of this study was to investigate the genetic structure of Iranian native horse breeds to compare genetic diversity and understanding the relationships between the populations using 11 ISAG microsatellite markers. For this reason, 565 samples of the Iranian Equestrian database from different ages ...
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The aim of this study was to investigate the genetic structure of Iranian native horse breeds to compare genetic diversity and understanding the relationships between the populations using 11 ISAG microsatellite markers. For this reason, 565 samples of the Iranian Equestrian database from different ages including the Iranian Arab Asil, Caspian, Darehshouri, Kurdish and Turkmen and 113 samples were used for each breed. The number of observed alleles for each locus was 7 to 19 alleles with an average of 10.81 alleles, and it was 19 for ASB17 and 7 for HTG4 locus with the highest and lowest observed alleles ranking respectively. The average observed heterozygosity from the largest to the lowest rank was, Turkmen (0.68±0.11), Caspian (0.67±0.07), Kurdish (0.66±0.06) Darehshouri (0.65±0.07), and Arab Asil (0.62±0.08). Population structure analysis with UPGMA method showed that Caspian and Kurdish populations were grouped as a unit cluster while the other populations grouped as a separate cluster. These results confirmed this hypothesis that the Caspian and Kurdish populations are close to the Nisa horses. In general, the results of this study indicate that the Iranian native horses have got a high genetic diversity, despite of populations have genetic similarity and the other hand genetic clustering of the populations is consistent with their geographic distances. The result of this study shows that the ISAG microsatellite markers are polymorphic and have more efficiency for assignment genetic diversity and genetic structure analysis of Iranian native horse breeds.
Samane Abuli; Gholam Reza Dashab; Mohammad Rokouei; Mehdi Vafaei valeh
Volume 17, Issue 1 , April 2015, , Pages 107-117
Abstract
The polymorphism in exon three of FABP4 gene and its association with growth traits of 45 Sistani (n=30) and Dashtiari (n=15) cattle were investigated. DNA extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. Animal genotypes were ...
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The polymorphism in exon three of FABP4 gene and its association with growth traits of 45 Sistani (n=30) and Dashtiari (n=15) cattle were investigated. DNA extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. Animal genotypes were determined based on polymerase chain reaction (PCR) products and their band size electrophoresed on agarose 2.8 percent resulted from enzyme digestion by NlaIII. The pattern of bands showed three genotypes including AA, AB and BB in two Sistani and Dashtiari breeds with frequency of 67, 30 and three percent and 73, 27 and zero percent, respectively. The frequency of A and B alleles in exon three of FABP4 in Sistani and Dashtiari breeds were 82 and 18 percent and 86.5 and 13.5 percent, respectively. Heterozygosity indices including Shannon index (I), Nei’s index, observed and expected heterozygosity in Sistani and Dashtiari population were 48, 30, 30 and 30 percent and 39, 11, 27 and 24 percent, respectively. The association between genotypes and growth-related traits were significant for body weights in six, nine and 12 months of age. Therefore, this locus can be considered as a candidate gene in breeding programs for describing the variation of growth traits after weaning age in calves.