Essa Dirandeh; Mohammad Kazemi Fard; Tannaz Saberifar
Volume 24, Issue 2 , July 2022, , Pages 227-235
Abstract
The occurrence and spread of inflammation can affect the quantity and quality of eggs in laying hens. Therefore, the aim of this study was to evaluate the effect of soy Gensitein (GEN)on systemic inflammatory status and performances of laying hens post-peak. The research was performed in a completely ...
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The occurrence and spread of inflammation can affect the quantity and quality of eggs in laying hens. Therefore, the aim of this study was to evaluate the effect of soy Gensitein (GEN)on systemic inflammatory status and performances of laying hens post-peak. The research was performed in a completely randomized design, during a period of about 8 weeks, using 80 laying hens of High Line W-36 strains (older than 43 weeks). Experimental treatments were control group (basal diet) and genistein group (basal diet+ 20mg GEN/kg of diet). At the end of experiment, five hens from each treatment were laughter and 50 g of liver sample were taken for gene expression of IL-1, IL-2, TNF-α, COX-1 and COX-2. Results showed that soy GEN decreased gene expression of IL-1 (245 fold), IL-2 (3.53 fold), IL-6 (2.68 fold), TNF-α (4.83 fold), COX-1 (3.92 fold) and COX-2 (1.73 fold) compared to the control group. Laying hens fed GEN diets had higher feed intake and better conversion ratio and had higher egg production rates than the control group.The results of this study showed that soy GEN could reduce inflammation in the liver of laying hens post-peak and improve production performances.
Golnaz Taasoli; Farrokh Kafilzadeh; Darab Ghadimi; Juan Loor; Micheal Ballou
Volume 22, Issue 4 , December 2020, , Pages 529-536
Abstract
The objective of this experiment was to study the effect of diet containing high (40.7 %) non fibrous carbohydrates on some inflammatory and native immunity related genes expression in lactating Jersey cows. Seven lactating multiparous (2.5 ± 0.5) Jersey cows with 71 ± 3 days in milk ...
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The objective of this experiment was to study the effect of diet containing high (40.7 %) non fibrous carbohydrates on some inflammatory and native immunity related genes expression in lactating Jersey cows. Seven lactating multiparous (2.5 ± 0.5) Jersey cows with 71 ± 3 days in milk and 28 ± 6.6 kg of average milk production were used. All cows received a control diet containing low (34.3 percentage of dry matter) non fibrous carbohydrates content for the first 14 days and then were were switched to a diet containing high (40.7 percentage of dry matter) non fibrous carbohydrates content for one week. Blood samples were collected on day 0 (After 14 days of feeding control diet) and days 4 and 7 after feeding high NFC diet. Relative gene expression of seven genes (IL1-β, TNF-α, MNDA, CD74, TLR2, SELECTIN, TGF1-β) were measured. The results showed that gene expression of the pro-inflammatory cytokines (IL1-β and TNF-α) and anti-inflammatory proteins (MNDA, D74, TLR2, SELECTIN, TGF1-β) was down regulated during feeding of high NFC diet (p < 0.05). These data indicated that feeding a high non-fibrous carbohydrates diet caused inflammation and affected immunity system in lactating Jersey cows.
Volume 18, Issue 4 , December 2016, , Pages 647-659
Abstract
Bovine mastitis is an inflammation disease of the mammary gland that impose considerable costs to the dairy industry. Regulatory mechanisms of this disease is complex and controlled by various gene regulatory elements and more studies are needed to better understand this disease. In the present study ...
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Bovine mastitis is an inflammation disease of the mammary gland that impose considerable costs to the dairy industry. Regulatory mechanisms of this disease is complex and controlled by various gene regulatory elements and more studies are needed to better understand this disease. In the present study aimed to better understand of regulatory elements involved in mastitis, milk samples of two groups of healthy and infected cows during time series of 0, 12, 24, 36 and 48 hours after contamination were collected. The miRNA-seq data obtained from the milk samples and using the advanced bioinformatics, novel miRNAs, their targets and probability functions, isomirs and novel miRNAs* were identified. The results led to the identification of 92 novel miRNA including 26 miRNAs with homologous and 66 miRNAs without homologous genes in other species. Investigation of the functional groups of predicted targets genes, confirmed the roles of new miRNAs in response to internal and external stimulations, apoptosis and production of immunoglobulin. Furthermore, 135 novel miRNAs were identified. Also, 493 novel isomeric sibling miRNAs (isomers) were discovered that immune related functions of these isomirs were demonstrated in some species like human and mouse. Identification of miRNAs target genes with associated functions in mastitis, including safety, apoptosis and inflammation, can indicated the possible regulatory roles of the identified miRNAs in mastitis.