Kobra Karimi; Mohammad taghi Beigi nassiri; Mahmood Nazari; khalil Mirzadeh
Volume 21, Issue 4 , January 2020, , Pages 431-439
Abstract
The present study was conducted to evaluate the effect of Tribulus Tresstrise (TT) herb on sex ratio of semen in Arabic Khouzestan ram using real time-qPCR technique using 18 rams in a completely randomized design with 3 treatments. The SRY and PLP genes were amplified to isolate the specific ...
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The present study was conducted to evaluate the effect of Tribulus Tresstrise (TT) herb on sex ratio of semen in Arabic Khouzestan ram using real time-qPCR technique using 18 rams in a completely randomized design with 3 treatments. The SRY and PLP genes were amplified to isolate the specific fragments of Y- and X- chromosome sequences. The treatments included: i) the control group (0% TT), ii) Diet containing 15 g/kg TT, iii) Diet containing 30 g/kg TT. Sperm sampling was taken from all rams at 10 month of age and blood sampling was performed at 8 and 10 month of age. The results showed that expression rate of SRY gene increased with increasing TT level and rams that received 30 g/kg TT diet had the highest SRY gene expression and PLP gene expression decreased with increasing TT level (p-value =0.004). There was positive correlation between Testosterone concentration and SRY gene expression at 8 (0.65) and 10 (0.59) month of age, and the relationship between PLP gene expression and Testosterone concentration was negative and -0.61 and -0.66 at 8 and 10 month of age, respectively (p-value= 0.006). The results indicated that adding Tribulus Tresstrise herb to the ram diet increases the SRY gene expression and also sperm containing Y chromosome. In other words, it increases the sex ratio toward male gens in Arabic Khozestan ram by increasing the androgen hormones.
Asghar Sedaghat; Mohammad Amir Karimi Torshizi; Shaban Rahimi
Volume 18, Issue 2 , June 2016, , Pages 273-286
Abstract
This study was conducted to evaluate the effect of camphor levels on performance, organ weight, testosterone and thyroid hormones, intestinal selective bacteria, and meat peroxidation of Japanese quail. Two-hundred 1-d chicks were allocated in 20 cages with five treatments and four replicates (10 chicks ...
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This study was conducted to evaluate the effect of camphor levels on performance, organ weight, testosterone and thyroid hormones, intestinal selective bacteria, and meat peroxidation of Japanese quail. Two-hundred 1-d chicks were allocated in 20 cages with five treatments and four replicates (10 chicks per each cage) using complete randomized block design. Treatments were including: 0, 62.5, 125, 250, and 500 ppm camphor which dissolved in soybean oil. Blood sample was taken then quails were slaughtered at 42-d of age. Results revealed camphor unaffected the carcass and organs weight. Also feed conversion ratio, feed intake, live body weight, and thyroid hormones unaffected by using camphor supplementation in the diet. Results showed that camphor could enhance libido by increasing the production and secretion of testosterone (P< 0.05). Dietary camphor had a significant effect on the intestinal microbial population, so that could reduce the aerobic bacteria and coliforms, whereas increased the lactic acid bacteria and spore former bacteria (P< 0.05). Meat malondialdehyde level has been also changed significantly in both sexes by camphor dietary supplementation, which increased by using 62.5, 125, and 250 ppm camphor (P< 0.05). According to the current results, using camphor is recommendable for successfully reproduction in male birds.