Hanie Shafigh; Malek Shakeri; Saeed Zeinoaldini; Hamid Kohram; Mehdi Zhandi; Morteza Moghbeli
Volume 18, Issue 3 , October 2016, , Pages 615-624
Abstract
The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain ...
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The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain rooster were used. Semen samples were collected, 2 times a week. After adding the samples of semen based on the lecithin extender, they were placed and preserved at 5 ° C. The treatments of 10 and 12.5 mg/L of Rosemary significantly improved the mobility, in addition, treatments of 10, 12.5 and 16.6 mg/L improved progressive motility and viability before freezing (p≤ 0.05). The lowest and highest motility rates were respectively found in treatments of 0 and 10 mg/L of essential oil of rosemary (p≤ 0.05). Functional integrity of the sperm plasma membrane increased with treatment of 10 mg/L of essential oil of rosemary in comparison to other groups, except 12.5 mg/L. Also treatments of 10, 12.5 and 16.6 mg/L significantly decreased the apoptosis. According to these results, it seems that adding the levels of 10 and 12.5 mg/L rosemary extract’ in semen, based on lecithin extender can improve rooster’s sperm quality after thawing.
Mohammad Amin Namazizadegan; Malek Shakeri; Mahdi Zhandi; Mojtaba Zaghari; Ramin Shahabi
Volume 18, Issue 1 , April 2016, , Pages 183-190
Abstract
The aim of the current study was to evaluate the effects of the guanidinoacetic acid (GAA) supplement on the semen freezability of broiler breeder roosters. Experiments were performed with twenty Ross commercial strain broiler breeder roosters in four treatments and five repeats in a complete randomized ...
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The aim of the current study was to evaluate the effects of the guanidinoacetic acid (GAA) supplement on the semen freezability of broiler breeder roosters. Experiments were performed with twenty Ross commercial strain broiler breeder roosters in four treatments and five repeats in a complete randomized design. Roosters were fed a diet containing 0% (control), 0.06%, 0.12% and 0.18% GAA supplement for eight weeks. Semen samples were collected weekly by abdominal massage. In order to equilibrate the temperature, semen samples were diluted with an extender and placed inside the refrigerator at 5 ° C. Samples were then packed into straws, exposed to nitrogen vapor and finally moved into liquid nitrogen containers. After freezing-thawing, the total and progressive motility, viability, morphology and membrane integrity of spermatozoa were assessed. The mean of total motility at the 0.12% and 0.18% levels were significantly higher than that of other groups (P