toktam sadat Vafa; heshmat Sepehri moghadam; Mozhdeh Emadi; Alireza Hasani Bafarani
Volume 21, Issue 4 , January 2020, , Pages 569-577
Abstract
To examine the effect of glycyrrhizic acid on lipid peroxidation and vital parameters of Holstein bull sperm after freeze-thawing process, semen samples were collected from four mature Holstein bulls twice a week using artificial vagina. Ejaculates were pooled in order to eliminate the individual ...
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To examine the effect of glycyrrhizic acid on lipid peroxidation and vital parameters of Holstein bull sperm after freeze-thawing process, semen samples were collected from four mature Holstein bulls twice a week using artificial vagina. Ejaculates were pooled in order to eliminate the individual effects of bull. Semen samples were divided into four equal groups (8 reps) including Zero (control), 20, 30 and 40 mg/ml of glycyrrhizic acid along with diluents based on egg yolk-citrate. Following cooling and equilibration stage, semen samples were stored in nitrogen tank for 30 days. After thawing procedure, level of malondialdehyde in sperm samples were measured by ELISA. Also, membrane integrity, motility and viability of sperm were examined. Statistical analysis was carried out using one-way ANOVA and post-hoc Tukey tests (p<0.05). According to the results, membrane integrity, motility and viability of sperm samples treated with concentration of 20, 30 and 40 mg/ml glycyrrhizic acid dose dependent manner significantly increased and level of malondialdehyde dose dependent manner significantly decreased ccompared to control groups (p<0.05). Therefore, use of glycyrrhizic acid in bull semen diluent can improve sperm vital parameters and decreases lipid peroxidation of sperm after freeze-thawing process.
Mohammad Razagh nia; Borhan Shokrollahi
Volume 21, Issue 2 , July 2019, , Pages 301-308
Abstract
In the current study, immunohistochemical localization of Ghrelin was investigated in placenta tissue of Holstein cows. In order to localization of Ghrelin, pregnancy content of 5 cows were collected in abattoir and transferred to the laboratory. After, placenta separation, paraffin blocks were prepared. ...
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In the current study, immunohistochemical localization of Ghrelin was investigated in placenta tissue of Holstein cows. In order to localization of Ghrelin, pregnancy content of 5 cows were collected in abattoir and transferred to the laboratory. After, placenta separation, paraffin blocks were prepared. Mouse monoclonal anti-ghrelin antibody as the primary antibody and donkey anti-rabbit IgG (HRP) Polyclonal antibody as the secondary antibody were used to localise ghrelin in bovine placenta immunohistochemically. In the present study, to set up the immunohistochemistry test, the ram's testicular sample that existence of ghrelin had previously been confirmed in it,, was used as a positive control. The results indicated that immunoprooxidase reaction was performed in positive control. Also, the serum of rabbit was used as negative control instead of primary antibody. The results demonstrated that the antibody was specific for ghrelin and no immunoprooxidase reaction was observed in negative control. The results showed that ghrelin is expressed in mono and syncytium multinuclear trophoblast cells of bovine placenta, so that the immuno-peroxidase reaction was observed in mono and syncytium multinuclear trophoblast cells in bovine placenta. In general, according to the results of the current study, it can be concluded that ghrelin is expressed in the bovine placenta tissue; however, future studies should focus on the precise role of the hormone in the placenta during pregnancy.
Hamid Amanlou; Hadi Shafiei; Tahereh Amirabadi Farahani; Farshid Kheiri; Najme Eslamian Farsuni
Volume 18, Issue 1 , April 2016, , Pages 27-38
Abstract
In order to compare the precision of predictions of NRC (2001) and CNCPS models, 17 Holstein fresh cows were assigned in a randomized complete block design to a balanced diet with NRC (2001) and CNCPS software. The predicted DMI, energy allowable milk and protein allowable milk by both models were compared ...
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In order to compare the precision of predictions of NRC (2001) and CNCPS models, 17 Holstein fresh cows were assigned in a randomized complete block design to a balanced diet with NRC (2001) and CNCPS software. The predicted DMI, energy allowable milk and protein allowable milk by both models were compared with observed values. The precision of the predictions of models was evaluated by calculation of R2, mean bias and root mean square prediction error (RMSPE). Both models predicted DMI lower than the observed values (P<0.01). The mean bias, RMSPE and R2 were 2.22, 2.84 and 0.48 for NRC (2001) and 3.18, 3.99 and 0.52 for CNCPS, respectively and CNCPS predicted the DMI 20 percent lower than the observed value. The observed milk production was 29.78 kg/d. predicted energy allowable milk by NRC (2001) and CNCPS were 25.68 and 22.32 kg/d, respectively. Predicted protein allowable milk with NRC (2001) and CNCPS were 25.80 and 28.37 kg/d, respectively and NRC (2001) predicted the milk production 14 percent lower than observed value. Given the importance of DMI in fresh cows seems NRC (2001) has more precision compared to CNCPS for estimating nutritional requirements.
Mahshid Mohammadpanah; Homayoun Farhangfar; Moslem Bashtani
Volume 17, Issue 2 , October 2015, , Pages 183-197
Abstract
Correlation between breeding value of crude milk (CM) and energy-corrected milk (ECM) was studied based on a total of 774,013 test day records belongs to 88,456 first-parity Holsteins dairy cattle calving during 1997-2009 in 165 herds (from six provinces). Genetic analysis of the traits was carried out ...
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Correlation between breeding value of crude milk (CM) and energy-corrected milk (ECM) was studied based on a total of 774,013 test day records belongs to 88,456 first-parity Holsteins dairy cattle calving during 1997-2009 in 165 herds (from six provinces). Genetic analysis of the traits was carried out by a fixed regression test day model. In the model, effects of herd, calving year, production month, production age, type of cow genotype (grade or pure Holstein), sperm type, polynomial function, as well as additive genetic and permanent environmental random effects of the cows were included. Mean breeding values as the genetic evaluation is undertaken based on two traits were statistically significant (P<0.0001). Correlation between breeding value of CM and ECM was the highest (0.92) when all the cows were selected based upon breeding value for ECM and was the lowest (0.27) as 10 top cows were selected based upon breeding value for ECM. It could be therefore concluded that in the case of using ECM instead of CM records, change in the ranking of the elite cows is appreciable.
Kamran Akbari Pabandi; Hamid Reza Mirzaei Alamouti
Volume 17, Issue 1 , April 2015, , Pages 119-129
Abstract
This experiment was conducted to investigate the effect of feeding frequency and oil supplementation to the diet on performance and feeding behavior of lactating dairy cows. Twenty four lactating Holstein cows, 12 primiparous, (BW= 626±58 Kg and DIM= 195±44 day) and 12 multiparous, (BW= ...
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This experiment was conducted to investigate the effect of feeding frequency and oil supplementation to the diet on performance and feeding behavior of lactating dairy cows. Twenty four lactating Holstein cows, 12 primiparous, (BW= 626±58 Kg and DIM= 195±44 day) and 12 multiparous, (BW= 617±25 Kg and DIM= 207±39 day) were randomly assigned to four diets contained 2.5 percent supplemental oil or no oil and feeding frequency of 3 or 1 time per day in a randomized complete block design with a 2×2 factorial arrangement. Frequency of feed delivery and oil supplementation had no effect on dry matter intake. Oil supplementation reduced slug feeding especially with feeding once per day (P<0.05). Feeding frequency and oil supplementation interaction tended to significant for milk yield (P= 0.09), as there was a numerical increase in milk yield in oil 3 time feeding with oil diet. The cows fed oil diet in 3 time feed delivery had lower milk protein (P<0.05), but milk fat increased with 1 time feeding and oil supplementation (P<0.05). The results showed that oil supplementation to mid lactating cows diet can decreased compromised effects of decreasing feeding frequency.