Mir Hossein Najafi; saeed zeinoaldini; Abolfazl Zali; mahdi Ganjkhanlou; Hossein Mohammadi
Volume 23, Issue 1 , March 2021, , Pages 73-82
Abstract
This study was conducted to investigate the effect of reducing dietary ratio of omega-6/omega-3 essential fatty acid (EFA) on dry matter intake, yield and milk composition, ovarian performance and blood concentrations of estradiol and progesterone using 24 multiparous Holstein cows. Experimental treatments ...
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This study was conducted to investigate the effect of reducing dietary ratio of omega-6/omega-3 essential fatty acid (EFA) on dry matter intake, yield and milk composition, ovarian performance and blood concentrations of estradiol and progesterone using 24 multiparous Holstein cows. Experimental treatments including different n-6 to n-3 EFA ratio; namely, 2.5, 4.5, or 6.5 parts of n-6 to 1 part of n-3 EFA. Dry matter intake and milk yield of cows were recorded daily. Also, body condition score and milk composition were recorded and ovarian activity was monitored by transrectal ultrasound scanning. Dry matter intake and milk composition were not affected by treatments. Daily milk yield was significantly higher in low and medium n- 6/n-3 FA ratio diets in comparison to high diet (P<0.05). Cows assigned to high n-6/n-3 FA ratio diet had greater plasma Haptoglobin concentrations compared to other diets (P<0.05). Results indicated that number of large follicles was greater in medium diet than high diet (P<0.05). Progestrone concentration was significantly decrease in high diet treatment (P<0.05). Results of this study indicate that modulating dietary n-6/n-3 FA ratio could lead to a higher milk yield and improve ovarian performance in fresh cows.
Touba Nadri; saeed zeinoaldini; Armin towhodi; Gholamhossein Riazi; Mahdi zhandi; Mohsen Sharafi
Volume 22, Issue 3 , September 2020, , Pages 471-477
Abstract
This study was designed to add the reduced glutathione to a lecithin nanoparticle-based extender and evaluate the quality of bull sperm after freezing and thawing. In the present study, the effect of four different levels of glutathione (0, 1, 2.5 and 5 mM) in extender-based on lecithin nanoparticles ...
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This study was designed to add the reduced glutathione to a lecithin nanoparticle-based extender and evaluate the quality of bull sperm after freezing and thawing. In the present study, the effect of four different levels of glutathione (0, 1, 2.5 and 5 mM) in extender-based on lecithin nanoparticles was evaluated. The lecithin nanoparticles were prepared and the particle size was reduced by using a sonicator device. During three weeks, 48 ejaculates from six Holstein bulls were collected and frozen. Properties evaluated after freezing and thawing were kinetic parameters (CASA), membrane activity (HOST), membrane integrity (eosin-nigrosine) and morphology (Hancock solution). The results showed that using 2.5 mM glutathione in the extender significantly increased total and progressive motility (63.38±1.5 and 43.1±1.1 respectively, P <0.05). The results of eosin-nigrosine staining and Host test showed that the highest viability and cell membrane functionality were related to 2.5 mM treatment (64.8±1.5 and 58.1±1.1 respectively) (p < 0.05). In general, 2.5 mM glutathione could improve the quality of bull sperm compared with other concentrations after freezing and thawing process. It seems that the 2.5 mM glutathione is optimum concentration for bull extender based on the lecithin nanoparticles.
Hojjat Baghshahi; Saeed Zeinoaldini; Ahmad Zare Shahneh; Saeid Esmaeilkhanian
Volume 21, Issue 3 , September 2019, , Pages 393-400
Abstract
The effect of addition of different concentrations of folic acid (vitamin B9) to the culture medium on in vitro maturation of bovine oocytes was investigated in two experiments. Optimization of bovine oocyte maturation medium is one of the strategies affecting quality of maturity and fertility, ultimately ...
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The effect of addition of different concentrations of folic acid (vitamin B9) to the culture medium on in vitro maturation of bovine oocytes was investigated in two experiments. Optimization of bovine oocyte maturation medium is one of the strategies affecting quality of maturity and fertility, ultimately resulting in increasing the quality of produced bovine embryos. In the 1st experiment, to specify the concentrations of folic acid and homocysteine, ovarian follicles were divided into two categories including follicles with more or less than eight mm of diameter. Then, the concentrations were measured in both follicle groups as well as a commercial culture medium. In the second experiment, bovine ovaries were transferred to the laboratory immediately after slaughter and the cumulus-oocyte complexes were collected by aspiration from 2-8 mm diameter follicles. Oocytes were randomly divided into four groups and different levels of folic acid (0, 10, 100 and 1000 ng/ml) were added to maturation medium based on the results of the first experiment. Following incubation of oocytes, the percentage of mature oocytes was determined by Hoechst staining. The concentrations of folic acid and homocysteine in follicles with diameter of less than eight mm was higher than those with diameter of greater than eight mm, as well as the culture medium Supplementation of maturation medium with 100 ng/ml folic acid increased the percentage of metaphase-II oocytes compared to the control (P <0.05). Considering the concentrations of folic acid and homocysteine in the medium, results showed that the addition of folic acid improves in vitro maturation of bovine oocytes.
homeira hemmati; saeed zeinoaldini; Ahmad Zare Shahneh; Amin Kazemizadeh; Alireza yousefi
Volume 21, Issue 2 , July 2019, , Pages 291-300
Abstract
Trend in body weight, thyroid hormones and some blood metabolites changes in broiler breeder hens after the peak egg production was studied. Blood samples from 20 Ross 308 broiler breeder hens, were investigated from 47 to 55 weeks of age. Plasma lipid profile including cholesterol, triglyceride, lipoprotein ...
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Trend in body weight, thyroid hormones and some blood metabolites changes in broiler breeder hens after the peak egg production was studied. Blood samples from 20 Ross 308 broiler breeder hens, were investigated from 47 to 55 weeks of age. Plasma lipid profile including cholesterol, triglyceride, lipoprotein LDL, lipoprotein HDL, and also glucose concentrations were measured every two weeks, and plasma concentrations of triiodothyronine (T3) and tetradothyronine (T4), aspartate aminotransferase (GOT) and alanine aminotransferase (GPT) were measured at the beginning and at the end of the experimental period. Results showed that body weight and plasma concentration of glucose, triglycerides, cholesterol, and LDL were increased, and concentrations of HDL were decreased (P<0.05) over the experimental period. Plasma T4 concentrations were decreased with age of the birds (P<0.05), while T3 concentrations were not affected. Plasma GOT and GPT enzymes activities were increased over the experiment period (P<0.05). In general, by increasing of age and subsequently body weight of breeder hens, several major changes have been occurred in blood metabolites and metabolic hormones which could reduce production and reproduction of broiler breeder flocks.
Hanie Shafigh; Malek Shakeri; Saeed Zeinoaldini; Hamid Kohram; Mehdi Zhandi; Morteza Moghbeli
Volume 18, Issue 3 , October 2016, , Pages 615-624
Abstract
The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain ...
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The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain rooster were used. Semen samples were collected, 2 times a week. After adding the samples of semen based on the lecithin extender, they were placed and preserved at 5 ° C. The treatments of 10 and 12.5 mg/L of Rosemary significantly improved the mobility, in addition, treatments of 10, 12.5 and 16.6 mg/L improved progressive motility and viability before freezing (p≤ 0.05). The lowest and highest motility rates were respectively found in treatments of 0 and 10 mg/L of essential oil of rosemary (p≤ 0.05). Functional integrity of the sperm plasma membrane increased with treatment of 10 mg/L of essential oil of rosemary in comparison to other groups, except 12.5 mg/L. Also treatments of 10, 12.5 and 16.6 mg/L significantly decreased the apoptosis. According to these results, it seems that adding the levels of 10 and 12.5 mg/L rosemary extract’ in semen, based on lecithin extender can improve rooster’s sperm quality after thawing.