Document Type : Research Paper


1 Departemant of Animal sciences, Faculty of Agriculture, Razi University, iran

2 Department of Animal Science, College of Agriculture, Razi University, Kermanshah, Iran

3 Depart. of Animal sciences, Faculty of Agriculture, Razi University

4 Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

5 Department of Animal sciences sari agricultural science and natural resources university.


Forty multiparous Holstein cows were used to investigate the effect of supplementary rumen protected Conjugated linoleic Acid (CLA) with injectable Se and vitamin E (E-Se) supplement on immune system in Holstein cows and their calve in a commercial dairy farm at Ghazvin province. The animals were divided in four experimental groups in a complete randomize design based on factorial. The experimental groups were: basal diet (Control), basal diet plus injectable E-Se Supplement, basal diet plus CLA 100 g/day per each cow) and basal diet plus CLA 100 g/day per each cow and Injectable E-Se Supplement. The dose of injectable E-Se supplement was 40 ml/cow. The injection has done muscularly in each cow in days, -21, 1, 14, 28 and 42 after calving. Blood Samples of cows collected at days of injection. Blood samples of calves collected at birthday pre feeding colostrum and 7 days after birth. The result showed CLA supplement had no significantly effect on mean concentration of Immunoglobulin G (IgG), Total antioxidant status (TAS) and Malondialdehyde (MDA) in calves on days 1 and 7 (P>0.05). Effect of injectable E-Se Supplement on TAS concentration of Calve's Plasma had was significant (P>.05). CLA and injectable E-Se Supplements had no significant effect on mean concentration of IgG, TAS and MDA of cow`s colostrum (P>0.05). The CLA and E-Se supplements had no significant effect on TAS concentration and hematology Parameters of dairy cow (P>0.05). Based on acquried result use of CLA supplement in transition period had no effect on immune system of cows and their calves. Supplementary injection of E-Se significantly improved total antioxidant status of newborn calves.


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