Document Type : Research Paper
Author
Assistant Professor
Abstract
This study was conducted to investigate the effects of chromium-methionine (Cr-Met) supplementation
on the blood and immune parameters of broiler under acute stress. A total of one hundred twenty six broilers were allocated to 3 treatments and 3 replicates (14 birds per replicate) in a completely randomized design. Treatments were: 1) negative control: i.e. basal diet without stress and Cr-Met, 2) positive control: basal diet with stress and without Cr-Met, 3) basal diet with stress and supplemented with 1000 ppb Cr-Met. Stress was induced from day 18 by addition of 1.5 mg dexamethasone per kg of the diet for one week followed by a withdrawal period until day 46. Blood samples were collected on day 25 and 46 of age. Results showed that stress increased blood glucose and lipid concentrations of serum (P < 0.05). The addition of Cr-Met decreased cholesterol, TG, LDL and VLDL on day 25 of age (P < 0.05). However, broilers fed Cr-Met had higher TG, LDL, VLDL and less HDL compared to other treatments on day 46 (P < 0.05). Administration of dexamethasone significantly elevated concentrations of white blood cell (WBC), heterophil (H), and heterophil to lymphocyte ratio (P < 0.05), while these parameters decreased by Cr-Met supplementation on day 25 (P < 0.05). In conclusion, supplemental Cr-Met improved broiler response to physiological stress during the acute stress period. However, broilers did not benefit from feeding Cr-Met supplementation after dexamethasone induced stress.
Keywords
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