Document Type : Research Paper

Author

Abstract

This study was carried out toinvestigate the effect ofmannan-oligosaccharide prebioticonperformance,egg quality, immune response, intestine ileum microflora and nutrient digestibility in laying hens fed various levels of crude protein. A total of 150 Hy-Line W-36 layinghens were assigned to a 2×3 factorial arrangement of treatments. Experimental diets consisted of 3 levels of crude protein (recommended level for strain, 90 and 95% of recommended level) and 2 levels of mannan-oligosaccharides (0 and 0.1% of diet). Reduction of crude protein to 10% of basal diet resulted in decrease of egg weight, egg production, egg mass and feed conversion ratio (P<0.01). Supplementation of mannan-oligosaccharide into the diet improved egg production and egg mass (P<0.05). Newcastle antibody titer, primary and second antibody response against sheep red blood cell increased by supplementation of mannan-oligosaccharides (P<0.05). Reduction of crude protein to 5% reduced blood serum triglycerides concentration (P<0.05). Reduction of crude protein to 5 and 10 % of diet caused increases in digestibility coefficients of organic matter and Reduction of crude protein to 10 % caused decline of crude fat digestibility (P<0.05). Dietary supplementation mannan-oligosaccharides increased crude fat digestibility and decreased ileal E. coli count (P<0.05). So, supplementation of mannan-oligosaccharide at levels of 0.1% of diet improved immune parameters and intestinal ileum microflora. Similarly, crude protein at levels of 15.68% of diet did not reduced performance parameters of layinghens from age 47 to 57 weeks.

Keywords

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