Document Type : Research Paper
Authors
Islamic Azad University Shahrekord Branch
Abstract
The aim of this research was to recognize genetic polymorphism in intron place of BMP15 gene and its relationship with growth and reproduction traits in Lori-Bakhtiari sheep breed using sequencing method. Blood samples of 80 ewes and 20 rams of Lori-Bakhtiari sheep from breeding station of Shahrekord were collected. DNA was extracted from whole blood and their quality was evaluated using 1% agarose gel electrophoresis. A fragment with the size of 356 bp from extracted DNA was amplified using polymerase chain reaction (PCR) with a pair of specific primers. The PCR products were sequenced. Sequenced result was compared with sequenced gene in world gene bank by Blast program in NCBI. Results founds only a deletion mutation (D75A) in this gene that in this mutation thymine with 41.2% frequency was deleted .This mutation wasn't effect on reproduction and growth traits. Of course, it's better than polymorphisms in other segments of this gene were studied and then decided about effect of this gene on reproduction and growth trait.
Keywords
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