Effect of ginger compared with vitamin E on in vitro culture of spermatogonial stem cells (SSCs) of Afshari sheep

doi:10.22059/jap.2017.62055

Document Type : Research Paper

https://doi.org/10.22059/jap.2017.62055

Abstract

The goal of this paper was to study the effect of ginger extracts and vitamin E on the performance of ovine Spermatogonial Stem Cells (SSCs). For this purpose, different concentrations of hydro and hydroethanolic extracts of ginger and vitamin E on viability, colony formation and expression of inhibiting (bcl2ll and bcl2)and inducing (bax) apoptosisgenes were studied. Spermatogonial Stem Cells (SSCs) were extracted from lamb testes with slaughterhouse origin using two steps enzymatic digestion method and enrichment by differential plating method. The characterization of SSCs was carried by alkaline phosphatase staining and expression of c-kit and oct-4 genes. Results have shown that the viability of SSCs was decreased significantly by using more than 800 µg/mL of hydroethanolic extract of ginger, in comparision with control group (P<0.05), while 800 µg/mL of hydro extract of ginger has been resulted to be the most viable cells, and increasing the vitamin E concentration (upto 100 µg/mL) resulted to be the most survival of SSCs. Expression of bax in effective concentration of hydro extract of ginger (150 µg/mL) and vitamin E (50 µg/mL) was decreased, but hydroethanolic extract of ginger (150 µg/mL) and higher concentration of hydro extract of ginger (more than 800 µg/mL) increased the expression od bax. Based on the results of current study, 150 µg/mL of aqueous extract of ginger or 50 µg/mL of vitamin E can be used to improve ovine SSC culture system.

Keywords

20.1001.1.20096776.1396.19.2.19.7

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Animal Production

Effect of ginger compared with vitamin E on in vitro culture of spermatogonial stem cells (SSCs) of Afshari sheep

References

References

Volume 19, Issue 2
August 2017
Pages 493-506

Effect of ginger compared with vitamin E on in vitro culture of spermatogonial stem cells (SSCs) of Afshari sheep

References

References

Volume 19, Issue 2August 2017Pages 493-506

Volume 19, Issue 2
August 2017
Pages 493-506