Document Type : Research Paper
Abstract
The present experiment conducted to investigate the effect of processing broiler litter with different levels of tannin extracted from pomegranate peel on in vitro rumen digestion and fermentation, rumen fungi and bacteria growth using completely randomized design in Ramin Agriculture and Natural Resources University of Khuzestan. Dietary treatments were adding pomegranate peel extract to broiler litter at levels 0, 5, 10, 15, 20, 25, 30 and 35 percent of dietary dry matter (DM). The gas and potential of gas production were constant with increasing level of pomegranate peel extract up to 20 and 15 percent, respectively. Rate of gas production was similar between treatments, except for diets containing 20 and 35 percent of pomegranate peel extract which decreased significantly (P<0.05). Increasing the level of pomegranate peel extract decreased rumen ammonia nitrogen concentration, but increased partitioning factor, microbial protein production and efficiency of microbial protein compared to control diet (P<0.05). Highest and lowest two-step crude protein digestibility was observed in diets containing 15 and 35 percentage pomegranate peel extract, respectively (P<0.05). After three days incubation of diets in specific rumen bacteria medium with increasing level of pomegranate peel extract, DM digestibility and ammonia nitrogen concentration decreased (P<0.05). Dry matter digestibility in all of investigated times were constant by incubation of treatments containing pomegranate peel extract in specific rumen fungi medium. According to the results, processing of broiler litter with pomegranate peel extract up to 25 percent of dry matter has potential to improve nitrogen metabolism in ruminants without any negative effects on nutrients digestibility.
Keywords
- Abarghoyi MJ and Rouzbehan Y (2015) The effects of several levels of grape pomace extract on in vitro intestinal digestibility of dairy cow. Animal science 28 (106): 13-28. (In Persian).
- Abarghoyi MJ, Rouzbehan Y and Zamiri MJ (2014) Effect of non-extracted and extracted pomegranate-peel on in vitro gas production parameters of inoculum of Ghezel sheep. Journal of Ruminant Research 2 (2): 37-59. (In Persian).
- AOAC (1990) Official Methods of Analysis, 15th edition. Association of Official Analytical Chemists, Washington, DC, USA.
- Azizi-Shotorkhoft A, Rouzbehan Y and Fazaeli H (2012) The influence of the different carbohydrate sources on utilization efficiency of processed broiler litter in sheep. Livestock Science 148: 249- 254.
- Broderick G and Kang JH (1980) Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and In vitro media. Journal of Dairy Science 54: 1176–1183.
- Elemam MB, Fadelelseed AM and Salih AM (2009) Growth performance, digestibility, N-balance and rumen fermentation of lambs fed different levels of deep-stack broiler litter. Journal of Animal and Veterinary Advances 4: 9-16.
- Frutos P, Raso M, Hervás G, Mantecón ÁR, Pérez V, Giráldez FJ (2004) Is there any detrimental effect when a chestnut hydrolysable tannin extract is included in the diet of finishing lambs? Journal of Animal Research 53: 127-136.
- Jolazadeh AR, Dehghan Banadaki M and Rezayazdi K (2014) Effects of treatment soybean meal with tannins extracted from pistachio hulls on performance and carcass quality of Holstein male calves. Journal of Ruminant Research 2 (1): 1-16. (In Persian).
- Jolazadeh AR, Dehghan-banadaky M and Rezayazdi K (2015) Effects of soybean meal treated with tannins extracted from pistachio hulls on performance, ruminal fermentation, blood metabolites and nutrient digestion of Holstein bulls. Animal Feed Science and Technology 203: 33–40.
- Makkar H (2003) Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research 49: 241-256.
- Makkar HPS (2000) Quantification of Tannins in Tree Foliage. A Laboratory Manual for the FAO/IAEA Co-ordinated Research Project on Use of Nuclear and Related techniques to Develop Simple Tannin Assays for Predicting and Improving the safety and Efficiency of Feeding Ruminants on Tanniniferous Tree Foliage. Joint FAO/IAEA of Nuclear Techniques in Food and Agriculture Animal Production and Health Sub-programme, FAO/IAEA Working Document. IAEA, Vienna, Austria.
- Makkar HPS (2010) In vitro screening of feed resources for efficiency of microbial protein synthesis. In: Vercoe PE, Makkar HPS, Schlink AC (Eds.), In vitro Screening of Plant Resources for Extra-nutritional Attributes in Ruminants: Nuclear and Related Methodologies. IAEA, Dordrecht, the Netherlands, pp. 107–144.
- McSweeney C, Palmer B, McNeill D and Krause D (2001) Microbial interactions with tannins: nutritional consequences for ruminants. Animal Feed Science and Technology 91: 83-93.
- Menke KH and Stingass H (1988) Estimation of the energetic feed value obtained from chemical analysis and In vitro gas production using rumen fluid. Animal Research and Development 28: 6-55.
- Mezzomo R, Paulino P, Detmann E, Valadares Filho S, Paulino M, Monnerat J, Duarte M, Silva L and Moura L (2011) Influence of condensed tannin on intake, digestibility, and efficiency of protein utilization in beef steers fed high concentrate diet. Livestock Science 141: 1-11.
- Mohammadabadi T, Chaji M and Tabatabaei S (2010) The effect of tannic acid on In vitro gas production and rumen fermentation of sunflower meal. Journal of Animal and Veterinary Advances 9: 277-280.
- Mohammadabadi T, Danesh Mesgaran M, Chaji M and Tahmasebi R (2012) Evaluation of the effect of fat content of sunflower meal on rumen fungi growth and population by direct (quantitative competitive polymerase chain reaction) and indirect (dry matter and neutral detergent fiber disappearance) methods. African Journal of Biotechnology 11: 179-183.
- Muhammed S, Acamovic CS and Stewart T (1995) Effects of tannic acid, ellagic acid, gallic acid and catechin on cellulose degradation by the rumen fungus Neocallimastix frontalis strain RE1. Journal of Animal Science 60: 550A.
- Orskov ER and McDonald I (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science (Cambridge) 92: 499- 503.
- Patra Ak (2012) Dietary Phytochemicals and Microbes. Springer Science & Business Media. pp. 237-256.
- Rezaeian M, Beakes GW and Chaudhry AS (2005) Relative fibrolytic activities of anaerobic rumen fungi on untreated and sodium hydroxide treated barley straw in In vitro culture. Anaerobe 11 (3): 163-175.
- SAS (2001) Statistical Analysis System: Users Guide, Statistics, version 8.2. SAS Institute. Carry, N.C., USA.
- Singh B, Chaudhary LC, Agarwal N and Kamra DN (2011) Effect of feeding Ficus infectoria leaves on rumen microbial profile and nutrient utilization in goats. Asian-Australian Journal of Animal Science 24 (6): 810 – 817.
- Tan H, Sieo C, Abdullah N, Liang J, Huang X and Ho Y (2011) Effects of condensed tannins from Leucaena on methane production, rumen fermentation and populations of methanogens and protozoa In vitro. Animal Feed Science and Technology 169: 185-193.
- Tilley JMA and Terry RA (1963) A two-stage technique for the in digestion of forage crops. Journal of the British Grassland Society 18: 104-111.
- Van Soest PJ, Robertson JB and Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber and non-starch carbohydrate in relation to animal nutrition. Journal of Dairy Science 74: 3583-3597.
- Yanez Ruiz DR, Moumen A, Martin Garcia AI and Molina Alaide E (2004) Ruminal fermentation and degradation patterns, protozoa population and urinary purine derivatives excretion in goats and whether fed diets based on two stage olive cake: effect of PEG supply. Journal of Animal Science 82: 2023–2032.
- Yildiz S, Kaya I, Unal Y, Aksu Elmali D, Kaya S, Censiz M, Kaya M and Oncuer A (2005) Digestion and body weight change in Tuj lambs receiving Oak (Quercus hartwissiana) leaves with and without PEG. Animal Feed Science and Technology 122: 159-172.