Document Type : Research Paper

Abstract

In order to investigate the effect of different levels of Saturieja khuzistanica powder on the  histomorphometric of testis, 36 native roosters (40 week age and BW 2039±293gr) was used. Three levels of Saturieja khuzistanica powder (0, 20 and 40g/kg) over 8 weeks was added to base diet. After eighth weeks, roosters were slaughtered and testes were removed from abdominal cavity. Tissue sections of testis after stained by Hematoxylin-Eosin were used to measure  number of primary spermatocyte, secondary spermatocyte, spermatid, Sertoli cells, number of tubules and testicular vessels using optical microscope. In treatment with 40gr Saturieja khuzistanica powder per kg diet, number and density of vessels in testicular section were decreased compared to the control group (P< 0.05). According to these results, adding 40 gr/kg Saturieja khuzistanica powder to diet will maintain Sertoli cells and also increase primary spermatocyte, secondary spermatocyte and spermatid in comparison with the control group.

Keywords

  1. احتشام­نیا ع، رضایی­نژاد ج، موسوی­زاده م و علیخانی کوبان (1390) بررسی فعالیت آنزیم پراکسیداز در برخی میوه ها با کاربرد اسانس­های گیاهی آویشن باغی، مورد و مرزه خوزستانی. فن­آوری تولیدات گیاهی (پژوهش کشاورزی). 11(2):42-33.
  2. حیدری م.ج (1393) بررسی تأثیر سطوح جیره­­ای پودر گیاه مرزه خوزستانی بر ویژگی­های منی خروس‌های بومی. پایان نامه ارشد، دانشکده کشاورزی دانشگاه لرستان.
  3. زارع شحنه ا،  اسدزاده و، آهنگر م و ادیب­مرادی م (1391) بررسی میکروسکوپی اثر مصرف ال-آرژنین بر بافت بیضه خروس. دومین کنفرانس تخصصی صنعت طیور قم. ص 20.
  4. ضمیری، م.ج(1385) فیزیولوژی تولید مثل. چاپ اول انتشارات حق شناص، ص 345.
  5. قاسمی ص(1390) تاثیر سطوح جیره­ای پودر مرزه بر عملکرد رشد، راندمان لاشه و تلفات مرغ­های گوشتی پرورش یافته در شرایط تنس سرمایی. دانشکده کشاورزی دانشگاه لرستان.
    1. 6. Aitken JR, Clarkson JS (1987) Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. Journal of Reproduction  and Fertility 81: 459-469.
    2.  7. Bagatell CJ,  Dahl  KD and  Bremner  WJ (1994) The direct pituitary effect of testosterone to inhibit gonadotropin secretion in men is partially mediated by aromatization to estradiol. Journal of Andrology 15(1): 15-21.
    3. 8. Brown NL, Baylé  JD,  Scanes  CG,  and  Follett  BK (1975) Chicken gonadotropins: Their effects on the testes of immature and hypophysectomized Japanese quail. Cell and Tissue Research 22(2): 299-260.
    4. 9. Case  GL,  He-Mo H  and  Elson GE (1995) Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterol-suppressive isoprenoids. Lipids 30: 357-359.
    5. 10. Chance B, Sies H, Boveris A (1979) Hydroperoxide metabolism in mammalian organs. Physiology Review 59: 527-605.
    6. 11. Cummins JM,  Jequier AM and Kan R (1994) Molecular biology of human male infertility – links with aging, mitochondrial genetics and oxidative stress. Molecular Reproduction and  Development 37:345-50.
    7. 12. Edwin  jA,  Tohanna  LM,  Tjeerdsma- van  B,  Zweatzer  C,  Sara A,  Burt  P  and Haog  S (2006) Structural requirements for the Antimicrobial of Carvacrol. Pub. Faculty of Veterinary Medicine, Utrecht  University Press.
    8.  13. Farsam H, Amanlou  M,  Radpour  MR, Salehinia A N, and Shafiee A(2004) Composition of the essential oils of wild and cultivated Satureja khuzistanica Jamzad from Iran. Flavors and Fragrance Journal 19(4): 308-310.
    9.  14. Haeri  S, Minaie  B, Amin  G, Nikfar  S, Khorasani  R, Esmaily H and Abdollahi  M (2006) Effect of Satureja khuzestanica essential oil on male rat fertility. Fitoterapia 77(7):
       495-499.
    10. 15. Jedlinska-Krakowska M, Bomba G, Jakubowski  K,  Rotkiewicz T,  Jana  B and Penkowski  A (2006)  Impact of oxidative stress and supplementation with vitamins E and C on testes morphology in rats.  Journal of  Reproduction  and Development 52(2): 203-9.
    11. 16. Lewis SE, Sterling ES, Young IS, and Thompson W (1997) Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertility Sterility 67:142-145.
    12. 17. Ochsendoerf FR (1999) Infections in the male genital tract and reactive oxygen species.  Human  Reproduction Update 5: 399-420.
    13. 18. Sanchez de Rojas V, Somoza B, Ortega T, Villar AM  and  Tejerina T (1999) Vasodilatory effect in rat aorta of eriodictyol obtained from Satureja obovata. Planta Med. 65: 234-8.
    14. 19. Santos  MRV,  Moreira FV,  Fraga BP,  De Souza DP,  Bonjardim LR and  Quintans-Junior, LJ ( 2011)  Cardiovascular effects of monoterpenes: A review. Revista Brasileira de Farmacognosia 21(4): 764-71.
    15. 20. Skinner  MK, Schlitz  SM,  and Tananis Anthony CA (1989) Regulation of Sertoli Cell Differentiated Function: Testicular Transferrin and Androgen-Binding Protein Expression. Endocrinology 124(6): 3015-3024.
    16. 21. Slamenová  D  Horváthová  ESramková  M,  and Marsálková  L (2007)  DNA-protective effects of two components of essential plant oils carvacrol and thymol on mammalian cells cultured in vitro. Neoplasma 54(2):108-120.
    17. 22. Smith R, Vantman D, Ponce J  (1996) Total antioxidant capacity of human seminal plasma. Human Reproduction 11:1655-60.
    18. 23. Turk G,  Atessahin A,  Sonmez M,  Yuce A and  Osman A (2007) Lycopene protects against cyclosporine A-induced testicular toxicity in rats. Theriogenology 67: 778-785.
    19. 24. Ward DN,  Bousfield GR,  and Moore KH (1991) Gonadotropins. In: Cupps PT, Reproduction in Domestic Animals. California Academic Press PP.25-67.
    20. 25. Zeman M,  Kosutzky J,  and Bobakova E (1986) Testosterone concentration in the seminal plasma Oemanf cocks. British Poultry Science 27: 261–266.
    21. 26. Zhang  X,  Berry WD,  McDaniel GR, Roland DA,  Liu P, Calvert C, and Wilhite R (1999) Body weight and semen production of broiler breeder males as influenced by crude protein levels and feeding regimens during rearing. Poultry Science 78(2): 190-196.