Document Type : Research Paper

Authors

• Jafar Pish Jang Aghajeri ¹
• Ghodrat Rahimi Mianji ²
• Hasan Hafezian ²
• Mohsen Gholizadeh ²

¹ Department of Animal Science, Islamic Azad University, Maragheh branch, Maragheh, Iran

² Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

Abstract

In the present study, the polymorphism of four loci including of TLR4, IL2, IgL and IFNγ, in 100 commercial broiler and layer chickens using PCR-RFLP technique and enzymes of Tsp509I, Sau96I and HphI were investigated. In TLR4 marker site, two alleles of C (two bands of 138 and 119 bp) and G (three bands of 119, 99 and 39 bp), In IL2 locus, two alleles of A (four bands of 465, 64, 40 and 31bp) and B (five bands of 454, 64, 40, 31 and 11 bp), in IgL locus, two alleles M (two bands of 173, 161 and two bands of 10 bp) and N (three bands of 161, 103,70 and two bands of 10 bp) and in IFNγ locus, two alleles E (one band of 129 bp) and F (two bands of 90 and 39 bp) were identified. In TLR4, IL2, IgL and IFNγ markers sites, the population structure of the total population including the Shannon information index 0.43, 0.62, 0.68 and 0.69, the Fixation index -0.19, 0.37, -0.18 and 0.17 and the observed heterozygosity index 0.32, 0.27, 0.58 and 0.41 were estimated, respectively. Regarding the presence of polymorphism at these sites and in the case of studying the immune responses of the observed genotypes for these sites, can be used as marker for genetic breeding programs of chickens for increase of resistance to disease.

Keywords

• Breeding
• Disease
• Molecular marker
• mutation
• PCR-RFLP

20.1001.1.20096776.1397.20.4.3.2