University of Tehran Press Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X 2008-6776 17 2 2015 09 23 Effect of zinc nano-complex on bull semen quality after freeze-thawing process Effect of zinc nano-complex on bull semen quality after freeze-thawing process 371 380 54040 10.22059/jap.2015.54040 FA Raana Jahanbin M.Sc. Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran Parisa Yazdanshenas M.Sc. Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran Mehdi Amin Afshar Assistant Professor, Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran Abdollah Mohammadi Sangcheshmeh Assistant Professor, Departments of Animal Science, Abourayhan Campus, University of Tehran, Pakdasht, Iran Hamid Varnaseri Assistant Professor, Nahadehaye Dami Jahed Co. Tehran, Iran Mohammad Chamani Associate Professor,, Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran Mohammad Hasan Nazaran Ehrare Hargh Company, Tehran, Iran Mohammad Reza Bakhtiyarizadeh Assistant Professor, Departments of Animal Science, Abourayhan Campus, University of Tehran, Pakdasht, Iran 0000-0001-5336-6987 Journal Article 2014 11 04 The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc Nano-complex and frozen. After thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. The proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. Zinc Nano-complex groups represented a higher plasma membrane functionality than that of control group. Moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc Nano-complex possessed higher mitochondrial activity as compared with the control group. Mitochondrial activity in 10-2 was higher than the 10-6 group. In conclusion, supplementation of zn Nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters. The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc Nano-complex and frozen. After thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. The proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. Zinc Nano-complex groups represented a higher plasma membrane functionality than that of control group. Moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc Nano-complex possessed higher mitochondrial activity as compared with the control group. Mitochondrial activity in 10-2 was higher than the 10-6 group. In conclusion, supplementation of zn Nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters.

https://jap.ut.ac.ir/article_54040_d0101505a6fd875232823d5d7aa63e70.pdf