Mohammad Ali Abbasi; shokoufe ghazanfari; seyed davood sharifi; Hassan Ahmadi Gavlighi
Articles in Press, Accepted Manuscript, Available Online from 21 August 2021
Abstract
The effect of thymus vulgaris essential oil (TEO) and rapeseed oil (RO) on performance, immune traits and intestinal morphology using 432 one-day-old Ross 308 male broiler as a (3×3) factorial experiment in a completely randomized design including different levels of TEO (zero, 300 and 500 ppm) ...
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The effect of thymus vulgaris essential oil (TEO) and rapeseed oil (RO) on performance, immune traits and intestinal morphology using 432 one-day-old Ross 308 male broiler as a (3×3) factorial experiment in a completely randomized design including different levels of TEO (zero, 300 and 500 ppm) and RO (1, 3 and 5 percentage) were evaluated with nine treatments and four replicates. Throughout the rearing period, broiler chickens receiving 3% gained more weight than 1% of RO and chickens receiving 300 ppm gained more weight than those without TEO (P˂0.05). The use of 3% RO + 300 ppm TEO increased the thigh percentage compared to 1% RO. At 1 and 5 percentage levels of RO, with increasing levels of TEO in the diet, blood cholesterol and LDL concentrations decreased and blood HDL increased (P <0.01). Levels of 3% and 5% of RO + 300 and 500 ppm of TEO in broiler chicken diets increased lymphocyte percentage and antibody titer against Newcastle disease and decreased heterophil percentage and heterophil to lymphocyte ratio of blood (P <0.05). Broiler chickens receiving diet containing TEO had the highest ratio of villi height to crypt depth (P <0.01). Based on the results, the level of 3% rapeseed oil + 300 ppm thyme essential oil in the diet of broiler chickens can be used to improve performance, immunity and biochemical traits of blood.
Touba Nadri; saeed zeinoaldini; Armin towhodi; Gholamhossein Riazi; Mahdi zhandi; Mohsen Sharafi
Volume 22, Issue 3 , Summer 2020, , Pages 471-477
Abstract
This study was designed to add the reduced glutathione to a lecithin nanoparticle-based extender and evaluate the quality of bull sperm after freezing and thawing. In the present study, the effect of four different levels of glutathione (0, 1, 2.5 and 5 mM) in extender-based on lecithin nanoparticles ...
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This study was designed to add the reduced glutathione to a lecithin nanoparticle-based extender and evaluate the quality of bull sperm after freezing and thawing. In the present study, the effect of four different levels of glutathione (0, 1, 2.5 and 5 mM) in extender-based on lecithin nanoparticles was evaluated. The lecithin nanoparticles were prepared and the particle size was reduced by using a sonicator device. During three weeks, 48 ejaculates from six Holstein bulls were collected and frozen. Properties evaluated after freezing and thawing were kinetic parameters (CASA), membrane activity (HOST), membrane integrity (eosin-nigrosine) and morphology (Hancock solution). The results showed that using 2.5 mM glutathione in the extender significantly increased total and progressive motility (63.38±1.5 and 43.1±1.1 respectively, P <0.05). The results of eosin-nigrosine staining and Host test showed that the highest viability and cell membrane functionality were related to 2.5 mM treatment (64.8±1.5 and 58.1±1.1 respectively) (p < 0.05). In general, 2.5 mM glutathione could improve the quality of bull sperm compared with other concentrations after freezing and thawing process. It seems that the 2.5 mM glutathione is optimum concentration for bull extender based on the lecithin nanoparticles.
Amin Kazemizadeh; Ahmad Zare Shahneh; Ali Reza Yousefi; Hasan Mehrabani Yeganeh; Zarbakht Ansari Pirsaraei
Volume 20, Issue 3 , Autumn 2018, , Pages 487-498
Abstract
The present study was conducted to investigate the effect of dietary Curcumin supplementation on histological parameters of testis in aged Ross 308 broiler breeder roosters. A total of twelve 48-week old broiler breeder roosters in a completely randomized design were randomly assigned to four treatments ...
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The present study was conducted to investigate the effect of dietary Curcumin supplementation on histological parameters of testis in aged Ross 308 broiler breeder roosters. A total of twelve 48-week old broiler breeder roosters in a completely randomized design were randomly assigned to four treatments and three replicates during a 13 weeks of experimental period. Treatments included no dietary Curcumin supplementation (control group), and daily supplementation of 10, 20, and 30 mg Curcumin/birds as mixed in the basal diet. At the end of the experimental period, all of the roosters were slaughtered, and testis tissue samples were collected. Testicular weight was higher in the roosters that daily received 30 mg Curcumin compared with the control group (P<0.05). Dietary supplementation of 20 and 30 mg Curcumin/day increased the diameter of seminiferous tubule compared to the control group (P<0.05). Seminiferous epithelium thickness was dose-dependently increased in Curcumin-supplemented birds compared to the control group (P<0.05). The number of spermatogonia cells was increased in all treatment groups compared to the control group (P<0.05). The number of Leydig cells was also increased in roosters received 20 and 20 mg Curcumin/day compared to the control birds (P<0.05). However, treatments did not affect the number of testis blood vessels (P>0.05). According to the results of the present study, dietary supplementation of 30 mg Curcumin/day/bird improves testis histological parameters in aged broiler breeder roosters.
sadegh hosseini; morteza mamuei; saleh tabatabaei vakili; mohsen sari
Volume 19, Issue 1 , Spring 2017, , Pages 245-253
Abstract
The objective of this experiment was to study the effects of dietary nano-selenium supplementation on the glutathione peroxidase activity and sperm quality and quantity parameters of Arabian rams. In this experiment, twelve Arabian rams with an average weight of 73±3 kg and age of two to four ...
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The objective of this experiment was to study the effects of dietary nano-selenium supplementation on the glutathione peroxidase activity and sperm quality and quantity parameters of Arabian rams. In this experiment, twelve Arabian rams with an average weight of 73±3 kg and age of two to four years were used. Animals were divided into three groups: the control group (without -nano Selenium) and two experimental groups that received 0.4 and 0.8 mg nano-selenium per kg dry matter, respectively. The results showed that significant increase in semen selenium concentration and semen glutathione peroxidase activity in group 0.8 mg nano-selenium compared with the control group (P<0.05), but there was no significant difference between 0.4 mg nano-selenium per kg supplementation and control group. The volume of ejaculate, sperm motility and viability increased in treatments compared with the control. Spermatozoa concentration was increased in group 0.4 mg nano-selenium per kg, but it was decreased in Group 0.8 mg nano-selenium per kg. The percentage of sperm abnormality significantly increased in control compared with experimental groups. According to the results of this research, supplementation of 0.4 mg Nano-selenium per kg dry matter in the diet, increased the glutathione peroxidase activity in semen and improve the quality and quantity characteristics of sperm.