hossein manafi rasi; Hossein Heidary chah baghi; salman Afshar; Narges Afshari pour
Volume 19, Issue 4 , Winter 2018, , Pages 819-828
Abstract
This study was carried out to investigate the effect of Ferulla alliacea resin and wire copper oxide on internal parasite control as well as growth performance of Baluchi fattening lambs. Towenty-five Baluchi lambs, aged 6 to 7 months and weighed 20 ± 5 kg on average, were selected as trial sample. ...
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This study was carried out to investigate the effect of Ferulla alliacea resin and wire copper oxide on internal parasite control as well as growth performance of Baluchi fattening lambs. Towenty-five Baluchi lambs, aged 6 to 7 months and weighed 20 ± 5 kg on average, were selected as trial sample. The experiment was done using completely randomized design with five groups as treatments replicated five times for 60 days. Treatments consisted of 1) control ( without additive), 2) Ferulla alliacea resin, 3) copper oxide wire, 4) combination of copper and Ferulla alliacea resin, 5) albendazole (anti parasite druge). During the experiment period, some parameters such as EPG (the number of eggs per gr of feces) and PCV (hematocrit equivalent) were measured at 0, 15, 30, 45 and 60 day of experiment, as well as weight gain weekly. According to the results, body weight changes were significant in 3th, 4th, 5th and 7th weeks (p<0.05). No significant difference was observed for PCV at all times. In addition, it appeared that copper oxide, Ferulla alliacea resin and albendasole had the same effect on decreasing EPG on 60th d. In addition,. The combination of Ferulla alliacea resin and copper oxide effectively reduced EPG in a shorter time (45th d) . The findings showed that Ferulla alliacea and copper oxide can be used as control of internal parasites in sheep, especially in organic systems.
Hossein Manafi Rasi; Mohammad Chamani; Salman Afshar
Volume 16, Issue 2 , Autumn 2015, , Pages 167-178
Abstract
This study was carried out to determine the potential of rumen microorganisms in samples taken from slaughterhouse to produce fibrolytic enzymes and to compare different methods for extraction, concentration and conservation of them. According to the results, the average specific activity of cellulase ...
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This study was carried out to determine the potential of rumen microorganisms in samples taken from slaughterhouse to produce fibrolytic enzymes and to compare different methods for extraction, concentration and conservation of them. According to the results, the average specific activity of cellulase and xylanase were 7.5 and 16.5 U/mg protein respectively. The application of homogenizing and sonication methods to extract rumen content enzymes showed that the former had better performance in the liberation of enzymes. To determine optimum time length for the maximum release of enzymes from solid phases, various time lengths including one, 1.5 and two min were examined. Based on this experiment, one minute appeared the best. To attain a high level of concentration of enzymes obtained from rumen liquor, various procedures were applied. The results indicate that freeze drying and precipitation of enzyme using ammonium sulphate were the best methods, while trichloroacetic acid precipitation turned out to be the most inappropriate method due to improper effect on enzymatic activity. In order to examine the effect of temperature and time length on enzymes activities, some methods including liquid nitrogen, freezing in -70° and freezing in -20° were applied. The comparison of different methods of enzymes conservation indicated that applying liquid nitrogen for long term as well as keeping in the freezer (-70°) for medium term purposes were the best.