amir karamzadeh-dehaghani; Armin towhidi; mahdi Zhandi; Naheed Mojgani
Volume 22, Issue 4 , December 2020, , Pages 659-688
Abstract
To investigate the effect of oral administration of prepared specific egg yolk antibody (IgY) against enterotoxigenic Escherichia coli K99 on growth performance, health, the prevalence of diarrhea of neonatal calves, an experiment was conducted using 60 newborn calves in a completely randomized design ...
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To investigate the effect of oral administration of prepared specific egg yolk antibody (IgY) against enterotoxigenic Escherichia coli K99 on growth performance, health, the prevalence of diarrhea of neonatal calves, an experiment was conducted using 60 newborn calves in a completely randomized design with two treatments. Calves were kept individually. Calves in the control group received only milk or colostrum for 30 days and calves in the treatment group received milk or colostrum with IgY against inactivated K99. During the experiment, health parameters and the prevalence of diarrhea were recorded daily, and growth performance was evaluated weekly. The results showed that average daily gain, starter dry matter intake and feed and milk efficiency were higher in the IgY group than the control group (P<0.05). The general health score in the IgY group was higher than control group and fecal and ear scores in this group were significantly lower than the control group (P<0.05). The count of fecal coliforms in the IgY group was decreased by approximately one log compared to the control group (P<0.05). The results of the present study showed that oral administration of IgY against enterotoxigenic Escherichia coli K99 has beneficial effects on health and average daily gain of suckling Holstein calves and It can be a good way to replace antibiotics.
Touba Nadri; saeed zeinoaldini; Armin towhodi; Gholamhossein Riazi; Mahdi zhandi; Mohsen Sharafi
Volume 22, Issue 3 , September 2020, , Pages 471-477
Abstract
This study was designed to add the reduced glutathione to a lecithin nanoparticle-based extender and evaluate the quality of bull sperm after freezing and thawing. In the present study, the effect of four different levels of glutathione (0, 1, 2.5 and 5 mM) in extender-based on lecithin nanoparticles ...
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This study was designed to add the reduced glutathione to a lecithin nanoparticle-based extender and evaluate the quality of bull sperm after freezing and thawing. In the present study, the effect of four different levels of glutathione (0, 1, 2.5 and 5 mM) in extender-based on lecithin nanoparticles was evaluated. The lecithin nanoparticles were prepared and the particle size was reduced by using a sonicator device. During three weeks, 48 ejaculates from six Holstein bulls were collected and frozen. Properties evaluated after freezing and thawing were kinetic parameters (CASA), membrane activity (HOST), membrane integrity (eosin-nigrosine) and morphology (Hancock solution). The results showed that using 2.5 mM glutathione in the extender significantly increased total and progressive motility (63.38±1.5 and 43.1±1.1 respectively, P <0.05). The results of eosin-nigrosine staining and Host test showed that the highest viability and cell membrane functionality were related to 2.5 mM treatment (64.8±1.5 and 58.1±1.1 respectively) (p < 0.05). In general, 2.5 mM glutathione could improve the quality of bull sperm compared with other concentrations after freezing and thawing process. It seems that the 2.5 mM glutathione is optimum concentration for bull extender based on the lecithin nanoparticles.
Amirhossein Nasiri; Armin Towhidi; Malek Shakeri; Mehdi Zhandi; Mehdi Dehghan banadaky
Volume 20, Issue 2 , August 2018, , Pages 329-337
Abstract
The insulin resistance and glucose, insulin, IGF-1 metabolism (somatotropic axis performance) were investigated in cows to receive (4 g yeast/d/head) (Probio-Sacc®, BioChem, GmbH, Germany) or not receive live yeast supplement from 21 d before expected date of calving under the hot months of summer ...
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The insulin resistance and glucose, insulin, IGF-1 metabolism (somatotropic axis performance) were investigated in cows to receive (4 g yeast/d/head) (Probio-Sacc®, BioChem, GmbH, Germany) or not receive live yeast supplement from 21 d before expected date of calving under the hot months of summer (THI = 82). Two groups of 6 periparturient Holstein cows were fed a diet without or with 4 g yeast/d/head (15*109 CFU/g) starting 21 d prepartum through 8 weeks postpartum to investigate the changes of blood concentrations of glucose, insulin, IGF-1 and glucose tolerance test (GTT). On d 60 postpartum, greater blood levels of glucose, insulin and insulin-like growth factor I were found in cows receiving yeast supplement than those receiving no yeast (P<0/05). Probiotic had not significant effect on GTT test. Overall, it appears that Probiotics would beneficially improve the blood concentrations of glucose, insulin, IGF-1 in day 60 postpartum (on DFS day) and improve somatotropic axis of dairy cows during the heat stress. Probiosac probiotic had positive effects on somatotropic axis and animal performance. It’s can be a good solution to increase dairy cow’s performance in heat stress condition.
Seyyed Mojtaba Mousavi; Armin Tohidi; Mehdi Zhandi; Abdollah Mohammadi-Sangcheshmeh; Ghasem Amou-abediny
Volume 18, Issue 3 , October 2016, , Pages 593-601
Abstract
The effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six Holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mOsml) ...
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The effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six Holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mOsml) and two levels of glycerol (5 and 7 %) .In general, semen samples were collected 36 times (from each bull, six times). After the initial evaluation of semen, samples were mixed together and assigned to each of treatments. After freezing and thawing process, parameters that were evaluated including; motion characteristics by CASA, viability, plasma membrane integrity and morphology. The results showed that frozen-thawed sperm in treatment of G7P300 had higher values than the other groups for total motility (69.50 %), progressive motility (48.89 %), lateral head displacement (3.69 µm/s), curvilinear velocity (168.80 µm/s) and straightness coefficient (61.89 %) (P≤0.05). In the treatments containing seven and five percent of glycerol and osmotic pressure of 350 mOsml, plasma membrane integrity (23.14 and 25.63 %, respectively) and sperm viability (58.70 and 64.60 %, respectively) were lower compared to other treatments (P≤0.05). But, in terms of morphology, G7P350 (92.34 %) and G5P350 (92.57 %) treatments were better than other treatments. The results of these experiment showed that the extender contained of seven percentage of glycerol with osmalarity of 300 or 250 mOsml was more efficient for cryopreservation of Holstein bull sperm.
Hanie Shafigh; Malek Shakeri; Saeed Zeinoaldini; Hamid Kohram; Mehdi Zhandi; Morteza Moghbeli
Volume 18, Issue 3 , October 2016, , Pages 615-624
Abstract
The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain ...
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The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain rooster were used. Semen samples were collected, 2 times a week. After adding the samples of semen based on the lecithin extender, they were placed and preserved at 5 ° C. The treatments of 10 and 12.5 mg/L of Rosemary significantly improved the mobility, in addition, treatments of 10, 12.5 and 16.6 mg/L improved progressive motility and viability before freezing (p≤ 0.05). The lowest and highest motility rates were respectively found in treatments of 0 and 10 mg/L of essential oil of rosemary (p≤ 0.05). Functional integrity of the sperm plasma membrane increased with treatment of 10 mg/L of essential oil of rosemary in comparison to other groups, except 12.5 mg/L. Also treatments of 10, 12.5 and 16.6 mg/L significantly decreased the apoptosis. According to these results, it seems that adding the levels of 10 and 12.5 mg/L rosemary extract’ in semen, based on lecithin extender can improve rooster’s sperm quality after thawing.
Mohammad Amin Namazizadegan; Malek Shakeri; Mahdi Zhandi; Mojtaba Zaghari; Ramin Shahabi
Volume 18, Issue 1 , April 2016, , Pages 183-190
Abstract
The aim of the current study was to evaluate the effects of the guanidinoacetic acid (GAA) supplement on the semen freezability of broiler breeder roosters. Experiments were performed with twenty Ross commercial strain broiler breeder roosters in four treatments and five repeats in a complete randomized ...
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The aim of the current study was to evaluate the effects of the guanidinoacetic acid (GAA) supplement on the semen freezability of broiler breeder roosters. Experiments were performed with twenty Ross commercial strain broiler breeder roosters in four treatments and five repeats in a complete randomized design. Roosters were fed a diet containing 0% (control), 0.06%, 0.12% and 0.18% GAA supplement for eight weeks. Semen samples were collected weekly by abdominal massage. In order to equilibrate the temperature, semen samples were diluted with an extender and placed inside the refrigerator at 5 ° C. Samples were then packed into straws, exposed to nitrogen vapor and finally moved into liquid nitrogen containers. After freezing-thawing, the total and progressive motility, viability, morphology and membrane integrity of spermatozoa were assessed. The mean of total motility at the 0.12% and 0.18% levels were significantly higher than that of other groups (P
Rasoul Karimi; Malek Shakeri; Mahdi Zhandi; Hossien Moravej; Haniyeh Banikamal; Abdollah Mohammadi-Sangcheshmeh; Mahdi Khodaei-Motlagh
Volume 17, Issue 2 , October 2015, , Pages 381-389
Abstract
The objective of this study was to evaluate the effect of the STO feeder layer on prepubertal Rhode Island Red rooster SSCs culture and proliferation in vitro. Testis cells from 30 prepubertal Rhode Island Red chicken (4-8 weeks of age), were individually separated and cultivated in the presence of ...
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The objective of this study was to evaluate the effect of the STO feeder layer on prepubertal Rhode Island Red rooster SSCs culture and proliferation in vitro. Testis cells from 30 prepubertal Rhode Island Red chicken (4-8 weeks of age), were individually separated and cultivated in the presence of bFGF and LIF growth factors on four well plates with two treatments and three replicats and five observations per each. SSCs colonies appeared on the 5th day of culture. The number of SSCs colonies, cells/colony and colony area was measured on days 7 and 10 for both treatments. The result of the colony assay on the 7th day revealed significantly higher colony numbers as well as higher cell number/colony and colony area on the STO surface compared to colonies grown on surfaces without a feeder layer (P≤0.05). In contrast, the results of the colony assay on day 10 had declined for both treatments, as compared to day 7. Also, the C-KIT gene was not expressed which is an indication that colonies might be composed of SSCs. In conclusion, these results indicate that the use of the STO feeder layer influences the SSCs proliferation and maintenance of the prepubertal roosters in short-term culture.
Hanieh Sadat Banikamal; Mahdi Zhandi; Malak Shakeri; Hossein Moravej
Volume 17, Issue 1 , April 2015, , Pages 29-37
Abstract
This study was conducted to investigate the effects of different levels of soybean lecithin on performance and blood lipids of broiler chicks. A total of 180 one day old Ross 308 broiler chicks were used in a completely randomized design with nine treatments (diets with 0, 0.2, 0.4, 0.6, 0.8, 1, 1.2, ...
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This study was conducted to investigate the effects of different levels of soybean lecithin on performance and blood lipids of broiler chicks. A total of 180 one day old Ross 308 broiler chicks were used in a completely randomized design with nine treatments (diets with 0, 0.2, 0.4, 0.6, 0.8, 1, 1.2, 1.4 and 1.6 percent of soybean lecithin) and four replicates for 47 days-rearing period. In order to evaluate the biochemical factors of blood including cholesterol, triglyceride, high density lipoprotein and low density lipoprotein, blood sampling from chickens were taken in day 41. The traits of weight gain, feed intake and feed conversion ratio were evaluated at the end of each period. The results showed that different levels of soybean lecithin had no significant effect on weight gain, feed intake and feed conversion ratio traits during each period. Increasing in the level of lecithin, decreased the concentration of cholesterol, triglyceride and low density lipoprotein and increased the high density lipoprotein concentration in blood plasma (P<0.05). In conclusion, dietary inclusion lecithin up to 1.6 percent, have no any effect on broiler performance, but could reduce cholesterol, triglyceride, and low density lipoprotein and increase high density lipoprotein in blood’s plasma.